McMicken College of Arts & SciencesMcMicken College of Arts & SciencesUniversity of Cincinnati

McMicken College of Arts & Sciences

Chemistry

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Laura B Sagle

Title: Assistant Professor
Office: 902 Crosley Tower
Tel: 513-556-1034
Email: laura.sagle@uc.edu
Web: http://www.thesaglegroup.com

Laura Sagle received her Ph.D. from UCSD in 2006 working with Professor Floyd Romesberg at the Scripps Research Institute. Her PhD work involved the development of a new site‐selective folding technique using infrared probes in proteins. Dr. Sagle then did postdoctoral research with Professor Paul Cremer at Texas A&M University, which investigated the role of small molecule stabilizers and
denaturants on protein‐mimetic polymers using microfluidics and sum frequency generation spectroscopy. From there, she carried out postdoctoral research with Professor Richard Van Duyne at Northwestern University. This work involved single nanoparticle tracking studies of solid supported bilayers, single nanoparticle LSPR biosensing, and incorporating LSPR and SERS substrates into microfluidic devices. Dr. Sagle joined the Chemistry Department at the University of Cincinnati in August of 2012 as an Assistant Professor.

The current research interests of the Sagle laboratory include the development of surface enhanced Raman spectroscopy (SERS) as a new single molecule protein folding technique, making improved biosensors from protein‐nanoparticle arrays, and using proteins as templates to make better nanomaterials.

Education

  • Bachelor of Science, University of California, Santa Cruz, Santa Cruz, CA, 1996 (Chemistry, Biochemistry Track).
  • Masters Degree in Chemistry, University of California, San Diego, La Jolla, CA, 2000 (Physical Chemistry).
  • Doctor of Philosophy, University of California, San Diego, La Jolla, CA, 2006 (Physical Chemistry).

Research Information

Research Interests

Single molecule protein folding using SERS, biosensing devices using protein-nanoparticle arrays (which will eventually be incorporated into microfluidic devices), and bionanomaterials using proteins as templates.

Research Support

  • (PI), Sagle, Laura, The Fabrication and Characterization of Nanoporous Alumina: A Pilot Project, National Aeronautics and Space Administration. (NNX13AR77H), $135,000. 09/30/2013 to 08/14/2016. Status: Active.
  • (PI), Laura Sagle, "Novel Nanoparticle Arrays For Improved Biosensing", UC LEAF SEED Award. $3,000. 02/01/2014 to 02/01/2014. Status: Completed.
  • (PI), Laura Sagle, Novel Plasmonic Platform For Cholera Toxin Binding, URC-Individual Faculty Grant. 11,427. 05/01/2015 to 08/15/2015. Status: Completed.
  • (Co-PI), Papautsky, Shanov, Alvarez, Dionysiou, Chae, Heineman, Sagle, Yadev, Haynes , Point-of-Care Sensors for Biomarkers of Environmental and Personal Exposure Assessment , UC Strategic Collaborative Research Grant. 150,000. BAD DATE to BAD DATE. Status: Active.
  • (Co-PI), Sagle, Steckl, Shanov, Gutmark, Heineman, "Renewable Energy Storage Devices Using Renewable Materials", UC Renewable Energy Program. $208,000. 03/01/2014 to 03/01/2015. Status: Completed.
  • (PI), Sagle, High Performance, Biocompatible Plasmonic Materials For Surface Enhanced Raman Spectroscopy (SERS), National Science Foundation: Division of Electrical, Communications and Cyber Systems. 403,471. BAD DATE to BAD DATE. Status: Pending.
  • (Co-PI), Merino and Sagle, Nucleus-Specific ROS Detection Using Nanoparticle SERS with Self-Cyclizing Agents, National Science Foundation: Chemistry of Life Processes Section. 399,094. BAD DATE to BAD DATE. Status: Pending.
  • (PI), Sagle, Laura, Novel Plasmonic Platform For Cholera Toxin Binding, UC's University Research Council. (URC Faculty Awards AY2014-15), $6,500.00. 05/01/2015 to 04/30/2016. Status: Active.
  • (Collaborator), Sagle, Laura; Tranter, Michael, DEVELOPMENT OF A MULTIPLEXED PLASMONIC NANOPARTICLE-BASED ASSAY TO IDENTIFY INHIBITERS OF HUR PROTEIN-RNA BINDING, UC's University Research Council. (URC Interdisciplinary Award AY2015-16), $25,000.00. 05/01/2016 to 04/30/2017. Status: Active.

Publications

Peer Reviewed Publications

  • Sagle, L. B., Cimatu, K., Litosh, V. A., Liu, Y., Flores, S., Chen, X., Yu, B., & Cremer, P. S. (2011). "The Methyl Groups of Trimethylamine N-oxide (TMAO) Orient Away From Hydrophobic Interfaces." J. Am. Chem. Soc., 133(46), 18707. [Link]
  • Cho, Younhee, Sagle, Laura, Iimura, Satoshi, Zhang, Yanjie, Kherb, Jaibir, Chilkoti, Ashutosh, Scholtz, J. Martin, & Paul S. Cremer (2009). “Hydrogen Bonding of ß-Spiral Structure is Stabilized in D2O”. J. Am. Chem. Soc., 131(42), 15188. [Link]
  • Sagle, Laura B., Zhang, Yanjie, Litosh, Vladislav, Chen, Xin, Cho, Younhee & Cremer, Paul S. (2009). "Investigating the Hydrogen Bonding Model of Urea Denatuation". J. Am. Chem. Soc., 131(26), 9304. [Link]
  • Cho, Younhee, Zhang, Yanjie, Carlson, Kim, Sagle, Laura, Chilkoti, A., & Cremer, P. S. (2008). "Effects of Hofmeister Anions on the Phase Transition Temperature of Elastin-Like Polypeptides". J. Phys. Chem. B, 112(44), 13765. [Link]
  • Chen, Xin, Sagle, Laura B., & Cremer, Paul S. (2007). "Urea Oreintation at the Protein Surface". J. Am. Chem. Soc., 129(49), 15104. [Link]
  • Zhang, Yanjie, Furyk, Steven, Sagle, Laura B., Cho, Younhee, Bergbreiter, David E. & Cremer, Paul S. (2007). "Effects of Hofmeister Anions on the LCSTs of PNIPAM as a Function of Molecular Weight". J. Phys. Chem. C, 111(25), 8916. [Link]
  • Weinkam, Patrick, Zimmermann, Jörg, Sagle, Laura B., Matsuda, Shigeo, Dawson, Philip E., Wolynes, Peter G. & Romesberg, Floyd E. (2008). "Characterization of the Alkaline Transitions in Ferricytochrome c Using Carbon-Deuterium Infrared Probes". Biochemistry, 47(51), 13470. [Link]
  • Sagle, Laura B., Zimmermann, Jörg, Dawson, Philip E., & Romesberg, Floyd E. (2006). "Direct and High Resolution Characterization of Cytochrome c Equilibrium Folding". J. Am. Chem. Soc., 128(44), 14232. [Link]
  • Sagle, Laura B., Zimmermann, Jörg, Matsuda, Shigeo, Dawson, Philip E., & Romesberg, Floyd E. (2006). "Redox-Coupled Dynamics and Folding in Cytochrome c". J. Am. Chem. Soc., 128(24), 7909. [Link]
  • Cremeens, Matthew E., Fujusaki, Hiroshi, Zhang, Yong, Zimmermann, Jörg, Sagle, Laura B., Matsuda, Shigeo, Dawson, Philip E., Straub, John E., & Romesberg, Floyd E. (2006). "Efforts Toward Developing Direct Probes of Protein Dynamics". J. Am. Chem. Soc., 128(18), 6028. [Link]
  • Sagle, Laura B., Zimmermann, Jörg, Dawson, Philip E., & Romesberg, Floyd E. (2004). "A High Resolution Probe of Protein Folding". J. Am. Chem. Soc., 126(11), 3384. [Link]
  • Paddock, M.L., Sagle, L., Tehrani, A., Beatty, J.T., Feher, G., Okamura, M. Y. (2003). "Mechanism of Proton Transfer Inhibition by Cd2+ Binding to Bacterial Reaction Centers: Determination of the pKa of Functionally Important Histidine Residues". Biochemistry, 42(32), 9626. [Link]
  • Adelroth, P, Paddock, ML, Sagle, LB, Feher, G, & Okamura, MY (2000). "Identification of the Proton Pathway in Bacterial Reaction Centers: Both Protons Associated with Reduction of Q(B) to Q(B)H(2) Share a Common Entry Point". Proceedings of the National Academy of Sciences of the United States of America, 97(N24), 13086. [Link]
  • Goldbeck, RA, Sagle, L, KimShapiro, DB, Flores, V, & Kliger, DS (1997). "Evidence for Heme-Heme Excitonic Coupling in the Soret Circular Dichroism of Hemoglobin". Biochemical and Biophysical Research Communications, 235(N3), 610. [Link]
  • Laura B. Sagle, Laura Ruvuna, Julia Bingham, Chunming Liu, Paul S. Cremer, & Richard P. VanDuyne (2012). Single Plasmonic Nanoparticle Tracking Studies of Solid Supported Bilayers with Ganglioside Lipids. Journal of the American Chemical Society, 134(38), 15832. [Link]
  • Sagle, Laura B., Ruvuna, Laura K., Ruemmele, Julia A., & Van Duyne, R. P. (2011). Advances In Localized Surface Plasmon Resonance Biosensing. Nanomedicine, 6(8), 1447. [Link]
  • Sarah Unser, Ian Campbell, Debrina Jana, and Laura B. Sagle. Direct Glucose Sensing in the Physiological Range Through Plasmonic Nanoparticle Formation. Analyst 2015, 140(2), 590-599.
  • Jana, D.; Matti, C.; He, J.; Sagle, L., “Capping Agent-Free Gold Nanostars Show Greatly Increased Versatility and Sensitivity for Biosensing”  Analytical Chemistry, 2015, 87(7) 3964-3972
  • He, J., Boegli, M., Bruzas, I., Lum, W., and Sagle, L., “Patterned Plasmonic Nanoparticle Arrays For Microfluidic And Multiplexed Biological Assays”  Analytical Chemistry, 2015, 87(22) 11407-11414

Book Chapter

  • Debrina Jana, Sarah Unser, Ian Bruzas, and Laura Sagle. Noble Metal Nanoparticles To Probe And Alter Biological Response. World Scientific Press, London, England, In Press. 

Invited Publication

  • Sagle, Laura B., Ruvuna, Laura K., Ruemmele, Julia A., & Van Duyne, R. P. (2011). "Advances in Localized Surface Plasmon Resonance Biosensing". Nanomedicine, 6(8), 1447. [Link]

Review

  • Unser, S., Bruzas, I., He, J., and Sagle, L., “Localized Surface Plasmon Resonance Biosensing: Current Challenges and Approaches”  Sensors, 2015, 15(7) 15684-15716

Presentations & Lectures

Invited Presentations

  • Laura Sagle, Xin Chen, Yanjie Zhang, Younhee Cho, and Paul S. Cremer (2009). “Infrared-Visible Sum Frequency Generation Spectroscopy as a Tool for Probing Small Molecule Denaturants and Stabilizers at the Protein-Water Interface” ACS National Meeting, Salt Lake City, UT.
  • Sagle, Laura B., Zimmermann, Jörg, Dawson, Philip E., and Romesberg, Floyd E. (2005). “Carbon-Deuterium Bonds as an Infrared Probe of Protein Dynamics, Local Dielectric and Folding” Rice University, Department of Physics and Astronomy Colloquia, Houston, TX.
  • Laura Sagle (02/06/2013). “Biophysics At The Lipid-Water Interface And Beyond” Departmental Colloquia, Physics Department, University of Cincinnati.
  • Laura Sagle (01/09/2013). “Protein Patterning Using Colloidal Lithography” Departmental Seminar, Rose-Hulman University, Terra Haute, IN.
  • Laura Sagle (04-2014). Biomaterials And Nanofabrication For Improved Localized Surface Plasmon Resonance Biosensing Oral Presentation, NASA, Moffett Field, CA.
  • Laura Sagle (04-2014). Improved Substrates For Plasmonic And SERS Applications Oral Presentation, Chemistry Department, Ohio University, Athens, OH.
  • Laura Sagle (04-2014). Improved Localized Surface Plasmon Resonance Biosensing Oral Presentation, Chemistry Department, Wright State University, Dayton, OH.
  • Laura Sagle (10-2014). Bionanomaterials For Improved Localized Surface Plasmon Resonance Biosensing Oral Presentation, Chemistry Department, Kenyon College, Gambier, OH.
  • Laura Sagle (12-2014). Bionanomaterials For Improved Localized Surface Plasmon Resonance Biosensing Oral Presentation, Chemistry Department, Marshall University, Huntington, WV.
  • Debrina Jana, Zohre Gorunmez, Jie He and Laura Sagle* (12-2014). Surface Enhanced Raman Spectroscopy (SERS) Of Core-Shell Structures With Different Shell Morphology Light-Driven Processes For Bio-Inspired Materials and Processes Workshop, Rice University, Houston, TX.
  • Laura Sagle (12/20/2015). Current Challenges And Approaches For Improved Localized Surface Plasmon Resonance Biosensing Invited Departmental Colloquia Seminar, Kent State University, Kent, Ohio.

Paper Presentations

  • Sarah Unser, Ian Campbell, Debrina Jana, and Laura Sagle (08-2014). Direct Glucose Sensing in the Physiological Range Through Plasmonic Nanoparticle Formation. National ACS Conference, San Francisco, CA.
  • Sarah Unser, Ian Campbell, Debrina Jana, and Laura Sagle (10-2014). Direct Glucose Sensing in the Physiological Range Through Plasmonic Nanoparticle Formation. CERM Conference, Pittsburgh, PA.
  • William Lum, Mario Vieweger, Haohan Zhao, and Laura Sagle (10-2014). Single Protein Patterning Using Hole Mask Colloidal Lithography. CERM Conference, Pittsburgh, PA.

Poster Presentations

  • Sagle, Laura B., Zimmermann, Jörg, Dawson, Philip E., and Romesberg, Floyd E. (2004). “Carbon-Deuterium Bonds as High Resolution IR Probes of Protein Folding” Protein Society Conference, San Diego, CA.
  • Paddock, M.L., Sagle, L., Tehrani, A., Beatty, J.T., Feher, G., Okamura, M. Y (2002). “Binding of Cd2+ and Ni2+ to the Reaction Center Displaces a Proton” Biophysical Society Conference, San Francisco, CA.
  • Haohan Zhao, William Lum, and Laura Sagle (09/12/2013). “Nano-Patterning By Hole-Mask Colloidal Lithography” ACS National Conference, Indianapolis, IN.
  • Sarah Unser, Jenifer Hatch, Ian Campbell, and Laura Sagle (09/11/2013). “Plasmonic Glucose Sensing With Collagen-Nanoparticle Arrays” ACS National Conference, Indianapolis, IN.
  • Debrina Jana, Jie He, and Laura Sagle (09/13/2013). “Probing The Inside Of Core-Shell Nanoparticles Using Surface Enhanced Raman Spectroscopy” ACS National Conference, Indianapolis, IN.
  • Carlos Matti, Debrina Jana, Jie He, Skyler Smith and Laura Sagle (08/14/2013). “Removal Of Capping Agents For Improved Biosensing” University of Cincinnati Department of Chemistry NSF-REU poster presentation, summer 2013, University of Cincinnati.
  • James Barnett, Sarah Unser, Laura Sagle (04/25/2013). “Applying Chemical Methods to the Devlopement of Better Biosenisng Technology” University of Cincinnati Undergraduate Research Symposium, poster presentation, Spring, 2013, University of Cincinnati.
  • William Lum, Haohan Zhao, Laura Sagle (04/25/2013). “Nano-Scale Patterning On Glass Substrates By Colloidal Lithography” University of Cincinnati Undergraduate Research Symposium, poster presentation, Spring, 2013, University of Cincinnati.
  • Ian Campbell, Sarah Unser, Laura Sagle (04/25/2013). “Bio-Sensing With Plasmonic Coupling Of Protien-Nanoparticle Composites” University of Cincinnati Undergraduate Research Symposium, poster presentation, Spring, 2013, University of Cincinnati.
  • Debrina Jana, Carlos Matti, Jie He, and Laura Sagle (03-2014). Improved biosensing using capping agent free Au nanostars PittCon Conference, Chicago, IL.
  • Debrina Jana, Carlos Matti, Jie He, and Laura Sagle (06-2014). Improved biosensing using capping agent free Au nanostars Gordon Research Conference -- Noble Metal Nanoparticles, South Hadley, MA.
  • Debrina Jana, Carlos Matti, Jie He, Emily Lehnhoff, and Laura Sagle (10-2014). Improved biosensing utilizing LSPR of noble metal nanoparticles both in solution and on solid substrate Oesper Symposium, University of Cincinnati.
  • Ian Bruzas, Sophia Melynk, and Laura Sagle (10-2014). Tunable nanoparticle arrays through templated electrodeposition of gold Oesper Symposium, University of Cincinnati.
  • Jendai Robinson, Jessica Koehne, Laura Sagle and Meyya Meyyappan (10-2014). The Fabrication and Characterization of Vertically Aligned Carbon Nanofibers Using Hole-Mask Colloidal Lithography towards Biosensing Applications Oesper Symposium, University of Cincinnati.
  • Jendai Robinson, Jessica Koehne, Laura Sagle and Meyya Meyyappan (09-2014). The Fabrication and Characterization of Vertically Aligned Carbon Nanofibers Using Hole-Mask Colloidal Lithography towards Biosensing Applications NOBCChE Conference, New Orleans, LA.
  • Sarah Unser, Jenifer Hatch, and Laura Sagle (03-2014). Plasmonic Glucose Sensing with Collagen-Nanoparticle Arrays PittCon Conference, Chicago, IL.
  • Sarah Unser, Ian Campbell, Debrina Jana, and Laura Sagle (10-2014). Direct Glucose Sensing in the Physiological Range Through Plasmonic Nanoparticle Formation Oesper Symposium, University of Cincinnati.
  • William Lum, Haohan Zhao and Laura Sagle* (06-2014). Extending Colloidal Lithography To Small Scales Gordon Research Conference -- Noble Metal Nanoparticles, South Hadley, MA.
  • Sarah Unser, Ian Campbell, Debrina Jana and Laura Sagle* (06-2014). Plasmonic Glucose Sensing In The Physiological Range Gordon Research Conference -- Bioanalytical Sensors, Newport, RI.

Lectures

  • (10/05/2012). Specific Ion Effects On The Water Solubility Of Macromolecules: PNIPAM And The Hofmiester Series. Guest Lecture for Graduate Thermodynamics Class, Crosley 304.

Honors & Awards

  • Maria Goeppert-Mayer Symposium, 1st Place Poster Prize, 2005 to 2005.
  • La Jolla Interfaces in Science Graduate Fellowship, 2002 to 2004.
  • Greater Advancement in Areas of National Need (GAANN) Graduate Fellowship, 1998 to 2001.
  • Novel Substrates For Single Molecule Biophysical Surface Enhanced Raman Spectroscopy (SERS), 05/15/2015 to 05/15/2016. 2014 Marion Milligan Mason Award for Women in the Chemical Sciences.

Experience & Service

Work Experience

  • 2012Assistant Professor, University of Cincinnati, Cincinnati, OH.
  • 2009 to 2012, Postdoctoral Research Associate, Van Duyne Group, Northwestern University, Evanston, IL.
  • 2006 to 2009, Postdoctoral Research Associate, Cremer Group, Texas A&M University, College Station, TX.
  • 2001 to 2006, Graduate Research Assistant, Romesberg Group, The Scripps Research Institute in California, La Jolla, CA.
  • 1998 to 2001, Graduate Research Assistant, Okamura/Feher Group, University of California, San Diego, La Jolla, CA.

Post Graduate training and Education

  • 2006 to 2009, Postdoctoral Research Associate, Cremer Group, Texas A&M University, College Station, TX.
  • 2009 to 2012, Postdoctoral Research Associate, Van Duyne Group, Northwestern University, Evanston, IL.

Student Advising

  • William Lum (Undergrad), Advisor. Status: In Progress
  • Jared Warncke (Undergrad), Committee Member. Status: Completed (12/05/2012)

Service

  • Committee Member, University of Cincinnati, Chemistry Department Graduate Admissions Comittee, 12/01/2012
  • Judge, Oakdale Elementary School Judges For Local Science Fair, 11/27/2012 to 11/27/2012
  • Peer Review/Referee, Journal of the American Chemical Society Peer Reviewer, 08/15/2012
  • Peer Review/Referee, NanoLetters Peer Reviewer, 09/15/2012
  • Peer Review/Referee, Journal Of Physical Chemistry C Peer Reviewer, 10/15/2012
  • Elected Member, RPT Committee, 11/15/2012
  • Member, Moral Committee, 08/15/2012
  • Committee Member, Chemistry Department Graduate Admissions Committee, 01-2014 to 04-2014
  • Committee Member, Chemistry Department Moral Committee, 08-2014 to 12-2014

Patents And Inventions

  • Patent Number: 61889689. “Colorimetric Glucose Sensing With Collagen-Nanoparticle Networks”. Laura Sagle and Sarah Unser. Submitted: 10/11/2013 (Co-Inventor).
  • Application Number: 116-006. Patterned Plasmonic Nanoparticle Arrays for Multiplexed, Microfluidic Biosensing Assays. Laura Sagle. (Inventor).

Other Experience and Professional Memberships

  • 10/01/2012, Society Of Applied Spectroscopy, . Full Member
  • 10/15/2012, ACS -- Local Cincinnati Chapter, . Member

Courses Taught

  • 15-CHEM-384 SURV PHYS CHEMISTRY
    Survey Of Physical Chemistry
    Level: Undergraduate
    Term: 12U

  • 15-CHEM-971 RESEARCH
    Undergraduate Research In Chemistry
    Level: Undergraduate
    Term: 12FS

  • -CHEM-3010 SURVEY PHYS CHEM
    Survey Of Physical Chemistry
    Level: Undergraduate
    Term: 13FS

  • Physical Chemistry Laboratory
    Physical Chemistry Laboratory
    Level: Undergraduate
    Term: 13FS

  • -CHEM-3010 SURVEY PHYS CHEM
    Survey Of Physical Chemistry
    Level: Undergraduate

  • Physical Chemistry Laboratory
    Physical Chemistry Laboratory
    Level: Undergraduate