McMicken College of Arts & SciencesMcMicken College of Arts & SciencesUniversity of Cincinnati

McMicken College of Arts & Sciences

Department of Chemistry

Department of Chemistry

Chemistry

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Patrick A Limbach

Title: Professor and Ohio Eminent Scholar
Office: 429 Rieveschl Hall
Tel: 513-556-1871
Email: pat.limbach@uc.edu
Web: http://bearcatms.uc.edu/

Pat Limbach is a Professor and Ohio Eminent Scholar at the University of Cincinnati. He is a bioanalytical chemist with research interests in mass spectrometry, ribonucleoprotein complexes and protein translation. After earning an undergraduate degree from Centre College in 1988, he studied under the direction of Dr. Alan G. Marshall at The Ohio State University. While there, his graduate research focused on instrumentation improvements to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. He received his PhD from OSU in 1992. He then took a postdoctoral position at the University of Utah working with Dr. James A. McCloskey. While in Utah, he worked in the area of RNA chemistry and nucleic acid mass spectrometry. In 1995, he joined the faculty at Louisiana State University and was promoted to Associate Professor in 1999. In 2001, he moved to his current position in Cincinnati. He has received numerous teaching and research awards including the Young Investigator Research Award from the American Society for Mass Spectrometry and the 2008 Cincinnati ACS Chemist of the Year.

Education

  • PhD, Ohio State University, 1992.
  • B.S., Centre College, Danville, KY, 1988 (Chemistry and Chemical Physics).

Research Information

Research Interests

mass spectrometry
modified nucleosides
transfer RNAs
structural investigation of nucleic acids
characterization of ribonucleoprotein complexes
assays for therapeutic oligonucleotides
nucleic acid biomarkers in humans

Research Support

  • (PI), Limbach, Patrick, Identification of Modified Nucleosides in Ribosomal Ribonucleic Acid, National Institute of General Medical Sciences. (R01-GM58843), 02/01/1999 to 01/31/2004. Status: Completed.
  • (co-PI), Limbach, Patrick, Mass Spectrometry Consortium for Materials and Medical Research, Ohio Board of Regents. (2001-HIF03), 06/20/2002 to 06/30/2004. Status: Completed.
  • (PI), Limbach, Patrick, Quantification of Macromolecules, Pfizer, Inc.. (SRS 00566), $127,485.00. 01/01/2007 to 06/30/2008. Status: Active.
  • (PI), Limbach, Patrick, Acquisition of a Hybrid Fourier Transform Mass Spectrometer for Proteomics, National Center for Research Resources. (1S10RR019900-01), $917,973.00. 04/25/2005 to 04/24/2006. Status: Closed.
  • (PI), Limbach, Patrick, Identification of Modified Nucleosides in Ribosomal Ribonucleic Acid, National Institute of General Medical Sciences. (3-R01-GM58843-04 NCE), $150,557.00. 02/01/2002 to 01/31/2004. Status: Closed.
  • (PI), Limbach, Patrick, Sequencing Transfer RNAs by Mass Spectrometry, National Science Foundation. (CHE-0910751), $450,000. 08/01/2009 to 07/31/2012. Status: Active.
  • (PI), Limbach, Patrick, Mass Spectrometry Consortium for Materials and Medical Research, Ohio Board of Regents. (2001-HIF03), $179,900.00. 06/20/2002 to 06/30/2004. Status: Closed.
  • (PI), Limbach, Patrick, Bioassays and Molecular Characterization of Microchip CE, National Institute of General Medical Sciences. (R01GM069547), $1,436,594.00. 01/01/2004 to 12/31/2008. Status: Closed.
  • (PI), Limbach, Patrick, The Development of Analytical Methods for Quantitation of Oligonucleotides, Girindus America Inc.. (SRS 005253), $140,000.00. 09/18/2007 to 03/18/2009. Status: Active.
  • (PI), Limbach, Patrick, Acquisition of an LC Mass Spectrometer for Nucleic Acids Research, National Center for Research Resources. (S10 RR027671), $500,000. 04/15/2010 to 04/14/2011. Status: Completed.
  • (PI), Limbach, Patrick, Chemistry Facilities Services, Chevron U.S.A. (SRS 006051), $12,000.00. 06/26/2008 to 12/31/2008. Status: Closed.
  • (PI), Limbach, Patrick, Mass Spectrometry of Ribosomal RNA: Protein Interactions, National Institute of General Medical Sciences. (R01 GM058843), $1,063,897. 09/30/2010 to 06/30/2014. Status: Active.
  • (PI), Limbach, Patrick, Sequencing Transfer RNAs by Mass Spectrometry, National Science Foundation. (CHE-0910751), $450,000.00. 08/01/2009 to 07/31/2012. Status: Closed.
  • (PI), Limbach, Patrick, Acquisition of an LC Mass Spectrometer for Nucleic Acids Research, National Center for Research Resources. (1 S10 RR027671-01), $500,000.00. 04/15/2010 to 04/14/2011. Status: Closed.
  • (PI), Limbach, Patrick, Mass Spectrometry of Ribosomal RNA: Protein Interactions, National Institute of General Medical Sciences. (R01 GM058843), $1,047,547.00. 09/30/2010 to 06/30/2014. Status: Active.
  • (PI), Limbach, Patrick, Quantification of Macromolecules, Pfizer, Inc.. (SRS 00566), 01/01/2007 to 06/30/2008. Status: Completed.
  • (PI), Limbach, Patrick, Comparative Analysis of Transfer RNAs by Mass Spectrometry, National Science Foundation. (CHE-1212625), $496,967.00. 08/15/2012 to 07/31/2015. Status: Active.
  • (PI), Limbach, Patrick, Acquisition of a Hybrid Fourier Transform Mass Spectrometer for Proteomics, National Center for Research Resources. (S10 RR019900), 04/25/2005 to 04/24/2006. Status: Completed.
  • (PI), Limbach, Patrick, Bioassays and Molecular Characterization of Microchip CE, National Institute of General Medical Sciences. (R01 GM069547), 01/01/2004 to 12/31/2008. Status: Completed.
  • (PI), Limbach, Patrick, The Development of Analytical Methods for Quantitation of Oligonucleotides, Girindus America Inc.. (SRS 005253), $90,000. 09/18/2007 to 03/18/2009. Status: Completed.
  • (PI), Limbach, Patrick, New Methods for the Analysis of Ribonucleic Acids, National Science Foundation. (CHE-0602413), $436,988. 07/01/2006 to 06/30/2010. Status: Completed.
  • (PI), Limbach, Patrick, Mass Spectrometry of Ribosomal RNA: Protein Interactions, National Institute of General Medical Sciences. (R01 GM058843), $1,050,772. 12/01/2004 to 08/31/2009. Status: Completed.
  • (PI), Limbach, Patrick, Single Cell Proteomics, National Science Foundation. (DBI-0137924), 04/01/2002 to 03/31/2005. Status: Completed.
  • (PI), Limbach, Patrick, Mass Spectrometry of Ribosomal RNA: Protein Interactions, National Institute of General Medical Sciences. (R01 GM058843-08S1), $102,341. 07/17/2009 to 11/30/2009. Status: Completed.
  • (PI), Patrick A Limbach, Comparative Analysis of Transfer RNAs by Mass Spectrometry, National Science Foundation. (CHE-1212625), $496,967. 08/15/2012 to 07/31/2015. Status: Active.
  • (PI), Limbach, Patrick, Mass Spectrometry of Ribosomal RNA: Protein Interactions, National Institute of General Medical Sciences. (R56 GM058843), $330,000. 09/01/2009 to 08/31/2010. Status: Completed.
  • (PI), Limbach, Patrick, Renovation of Chemistry Facilities at the University of Cincinnati, National Science Foundation. (CHE-0962996), $1,200,000. 09/15/2010 to 08/31/2013. Status: Active.

Publications

Peer Reviewed Publications

  • Mandal, D., Köhrer, C., Su, D., Russell, S.P., Krivos, K., Castleberry, C.M., Blum, P., Limbach, P.A., Söll, D., RajBhandary, U.L. “Agmatidine, a modified cytidine in the anticodon of archaeal tRNAIle, base pairs with Adenosine but not with Guanosine”, Proceedings of the National Academy of Sciences, USA 107 (2010) 2872-2877. PMCID: PMC2840323.
  • Krivos, K., Limbach, P.A. “Sequence Analysis of Peptide:Oligonucleotide Heteroconjugates by Electron Capture Dissociation and Electron Transfer Dissociation”, Journal of the American Society for Mass Spectrometry 21 (2010) 1387-97. PMID: 204335485.
  • Anton, B.P.; Russell, S.P.; Limbach, P.A.; Kasif, S.; Raleigh, E.A.; Roberts, R.J. “Functional Characterization of the YmcB and YqeV tRNA Methylthiotransferases of Bacillus subtilis”, Nucleic Acids Research 38 (2010) 6195-205. PMCID: PMC2952846
  • Nikcevic, I.; Wyrzykiewicz, T.K.; Limbach, P.A.; “Detecting Low-level Synthesis Impurities in Modified Phosphorothioate Oligonucleotides Using Liquid Chromatography-High Resolution Mass Spectrometry”, International Journal of Mass Spectrometry 304 (2011), 98-104.
  • Castleberry, C. M.; Limbach, P.A.; “Relative Quantitation of Transfer RNAs Using Liquid Chromatography-Mass Spectrometry (LC-MS) and Signature Digestion Products”, Nucleic Acids Research 38 (2010) e162. PMCID: PMC2938229
  • Nikcevic, I.; Piruska, A.; Wehmeyer, K.R.; Seliskar, C.J.; Limbach, P.A.; Heineman, W.R., “Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser induced fluorescence detection”, Electrophoresis 31 (2010) 2796-803. PMCID: PMC3031587.
  • Easter, R.N.; Kröning, K.K; Caruso, J. A.; Limbach, P.A.; “Separation and detection of oligonucleotides by hydrophilic interaction liquid chromatography (HILIC) - inductively coupled plasma mass spectrometry (ICPMS)”, Analyst 135 (2010) 2560-2565. PMID: 20830328
  • Addepalli, B.; Limbach, P.A.; Hunt, A.G “Disulfide Linkage in a CCCH Zinc Finger Motif of Arabidopsis CPSF30 Ortholog”, FEBS Letters 584 (2010) 4408-4412. PMID: 20888817
  • Currie, C.A.; Shim, J.S.; Lee, S.H.; Ahn, C.; Limbach, P.A.; Halsall, H.B.; Heineman, W.R. “Comparing Polyelectrolyte Multilayer-coated PMMA Microfluidic Devices and Glass Microchips for Electrophoretic Separations”, Electrophoresis 30 (2009) 4245-4250. PMCID: PMC2847454.
  • Castleberry, C.M.; Lilleness, K.; Baldauff, R.; Limbach, P.A. “Minimizing 18O/16O Back-exchange in the Relative Quantification of RNAs”, Journal of Mass Spectrometry 44 (2009) 1195-1202. PMID: 19484804.
  • Hossain, M.; Limbach, P.A. “Multiple Endonucleases Improve MALDI-MS Signature Digestion Product Detection of Bacterial Transfer RNAs”, Analytical and Bioanalytical Chemistry 394 (2009) 1125-1135. PMID: 19104781
  • Hamburg, D.-M.; Suh, M.-J.; Limbach, P.A. “Limited Proteolysis of the Ribosome is Affected by Subunit Assembly”, Biopolymers 91 (2009) 410-422. PMCID: PMC2936250.
  • Hossain, M.; Limbach, P.A.; “Mass Spectrometry-Based Detection of Transfer RNAs by Their Signature Endonuclease Digestion Products” RNA 13 (2007) 295-303. PMCID: PMC1781365.
  • Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "Frontal analysis in microchip capillary electrophoresis: a simple and accurate method for determination of protein-DNA dissociation constant". Electrophoresis (2007), 28, 837-842.
  • Gong, M.; Wehmeyer, K.R.; Stalcup, A.M.; Limbach, P.A.; Heineman, W.R. "Study of injection bias in a simple hydrodynamic injection in microchip capillary electrophoresis". Electrophoresis (2007), 28, 1564-1571.
  • Nikcevic, I.; Lee, S.-H.; Piruska, A.; Ahn, C.; Ridgway, T.H.; Limbach, P.A.; Wehymeyer, K.R.; Heineman, W.R.; Seliskar, C.J. "Characterization and performance of injection molded poly(methylmethacrylate) microchips for capillary electrophoresis". J. Chrom. A (2007), 1154, 444-453.
  • Suh, M.J.; Pourshahian, S.; Limbach, P.A. "Developing Limited Proteolysis and Mass Spectrometry for the Characterization of Ribosome Topography". J. Am. Soc. Mass Spectrom. (2007), 18, 1304-1317.
  • Duderstadt, R.E.; Tsuie, B.M.; Macha, S.F.; Limbach, P.A. "Investigation of Single-Site Zirconium Azaborolinyl Complexes by Laser Desorption Ionization Time-of-Flight Mass Spectrometry". Anal. Chim. Acta (2007), 596, 124-131.
  • Mecomber, J.S.; Stalcup, A.M.; Hurd, D; Halsall, H.B.; Heineman, W.R.; Seliskar, C.J.; Wehmeyer, K.R.; Limbach, P.A. "Analytical Performance of Polymer-Based Microfluidic Devices Fabricated by CNC Machining". Anal. Chem. (2006), 78, 936-941.
  • Carr, J.F.; Hamburg, D.-M.; Gregory, S.T.; Limbach, P.A.; Dahlberg, A.E. "Effect of Streptomycin-resistance Mutations on the Post-translational Modification of Ribosomal Protein S12". J. Bacteriol. (2006), 188, 2020-2023.
  • Meng, Z.; Limbach, P.A. "Mass Spectrometry of RNA: Linking the Genome to the Proteome". Briefings in Functional Genomics & Proteomics (2006), 5, 87-95.
  • Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Arias, F.; Heineman, W.R. "On-line Sample Preconcentration using Field-Amplified Stacking Injection in Microchip Capillary Electrophoresis". Anal. Chem. (2006), 78, 3730-3737.
  • Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "On-line Sample Preconcentration by Sweeping with Dodecyltrimethylammonium Bromide in Capillary Zone Electrophoresis". J. Chrom. A (2006), 1125, 263-269.
  • Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "Unlimited-volume Electrokinetic Stacking Injection in Sweeping Capillary Electrophoresis Using a Cationic Surfactant". Anal. Chem. (2006), 78, 6035-6042.
  • McLachlan, A.; Borchers, M.; Velayutham, P.; Wagner, M.; Limbach, P.A. "Characterizing the Reproducibility of a Protein Profiling Method for the Analysis of Mouse Bronchoalveolar Lavage Fluid". J. Proteome Res. (2006), 5, 3059-3065.
  • Wetherill, Y.B.; Hess-Wilson, J.K.; Comstock, C.E.S.; Shah, S.A.; Buncher, C.R.; Sallans, L.; Limbach, P.A.; Schwemberger, S.; Babcock, G.F.; Knudsen, K.E. "Bisphenol A facilitates bypass of androgen ablation therapy in prostate cancer". Mol Cancer Therapeutics (2006), 5, 3181-3190.
  • Tummala, R.; Limbach, P.A. "Effect of Sodium Dodecyl Sulfate Micelles on Peptide Mass Fingerprinting by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry". Rapid Commun. Mass Spectrom. (2005), 18, 2031-2035.
  • Meng, Z.; Limbach, P.A. "Quantitation of Ribonucleic Acids Using 18O Labeling and Mass Spectrometry". Anal. Chem. (2005), 77, 1891-1895.
  • Tu, J.; Halsall, H.B.; Seliskar, C.J.; Limbach, P.A.; Arias, F.; Wehmeyer, K.R.; Heineman, W.R. "Estimation of log Pow Values for Neutral and Basic Compounds by Microchip Microemulsion Electrokinetic Chromatography with Indirect Fluorimetric Detection". J. Pharm. Biomed. Anal. (2005), 38, 1-7.
  • Mecomber, J.S.; Hurd, D.; Limbach, P.A. "Enhanced machining of micron-scale features in microchip molding masters by CNC milling". Int. J. Machine Tools Manufacture (2005), 45, 1542-1550.
  • Tummala, R.; Green-Church, K.B.; Limbach, P.A. "Interactions Between Sodium Dodecyl Sulfate Micelles and Peptides During Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) of Proteolytic Digests". J. Am. Soc. Mass Spectrom. (2005), 16, 1438-1446.
  • Meng, Z.; Limbach, P.A. "Shotgun Sequencing Small Oligonucleotides by Use of Nozzle-Skimmer Dissociation and Electrospray Ionization Mass Spectrometry". Eur. J. Mass Spectrom. (2005), 11, 221-229.
  • Meng, Z.; Limbach, P.A. "Shotgun Sequencing Small Oligonucleotides by Use of Nozzle-Skimmer Dissociation and Electrospray Ionization Mass Spectrometry". Eur. J. Mass Spectrom., 11, 221-229.
  • Currie, C.C.; Heineman, W.R.; Halsall, H.B.; Seliskar, C.J.; Limbach, P.A.; Arias, F.; Wehmeyer, K.R. "Estimation of pKa Values Using Microchip CE Coupled with Indirect Fluorescence Detection". J. Chrom. B (2005), 824, 201-205.
  • Tummala, R.; Limbach, P.A. "Serum Protein Profiling Using Surfactant-Aided Matrix Assisted Laser Desorption/Ionization Mass Spectrometry". Anal. Chim. Acta (2005), 551, 137-141.
  • Meades, G.D. Jr.; McLachlan, A.; Sallans, L.; Limbach, P.A.; Frankel, L.K.; Bricker, T.M. "Association of the 17 kDa Protein with Photosystem II in Higher Plants". Biochemistry (2005), 44, 15216-15221.
  • Piruska, A.; Nikcevic, I.; Lee, S.-H.; Ahn, C.; Heineman, W.R.; Limbach, P.A.; Seliskar, C.J. "The Autofluorescence of Plastic Materials and Chips Under Laser Irradiation". Lab Chip (2005), 5, 1348-1354.
  • Suh, M.-J.; Hamburg, D.-M.; Gregory, S.T.; Dahlberg, A.E.; Limbach, P.A. "Extending Ribosomal Protein Identifications to Unsequenced Bacterial Strains Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry". Proteomics (2005), 5, 4818-4831.
  • Meng, Z.; Simmons-Willis, T.A.; Limbach, P.A. "The Use of Mass Spectrometry in Genomics". Biomolecular Engineering (2004), 21, 1-13.
  • Suh, M.-J.; Limbach, P.A. "Investigation of Protein Isolation Methods Suitable for the Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Analysis of Ribonucleoprotein Complexes". Eur. J. Mass Spectrom (2004), 10, 89-99.
  • Meng, Z.; Limbach, P.A. "RNase Mapping of Intact Nucleic Acids by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (ESI-FTICRMS) and 18O Labeling". Int. J. Mass Spectrom (2004), 234, 37-44.
  • Cameron, D.M.; Gregory, S.T.; Thompson, J.; Suh, M.-J.; Limbach, P.A.; Dahlberg, A.E. "Thermus thermophilus L11 Methyltransferase, PrmA, is Dispensable for Growth and Preferentially Modifies Free Ribosomal Protein L11 Prior to Ribosome Assembly". J. Bacteriol (2004), 186, 5819-5825.
  • Berhane, B.T.; Limbach, P.A. "Functional Microfabricated Sample Targets for Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Analysis of Ribonucleic Acids". Anal. Chem. (2003), 75, 1997-2003.
  • Dominick, W.D.; Berhane, B.T.; Mecomber, J.S.; Limbach, P.A. "Covalent Immobilization of Proteases and Nucleases to Poly(methylmethacrylate)". Anal. Bioanal. Chem (2003), 376, 349-354.
  • Berhane, B.T.; Limbach, P.A. "Stable Isotope Labeling for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Post-Source Decay Analysis of Ribonucleic Acids". J. Mass Spectrom (2003), 38, 872-878.
  • Robins, C.R.; Limbach, P.A. "The Use of Nonpolar Matrices for Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Analysis of High Boiling Crude Oil Fractions". Rapid Commun. Mass Spectrom (2003), 17, 2839-2845.
  • Hassett, D.J.; Limbach, P.A.; Hennigan, R.F.; Klose, K.E.; Hancock, R.E.W.; Platt, M.D.; Hunt, D.F. "Bacterial Biofilms of Importance to Medicine and Bioterrorism: Proteomic Techniques to Identify Novel Vaccine Components and Drug Targets". Exp. Opin. Biol. Therapy (2003), 3, 1201-1207.
  • Robins, C.L.; Macha, S.F.; Vandell, V.E.; Limbach, P.A. "LDI- and MALDI-Fourier Transform Ion Cyclotron Resonance (FTICR) Mass Spectrometry Analysis of Hydrocarbon Samples". In Analytical Advances in Hydrocarbon Research; Hsu, S., Ed(s); New York, N.Y.: Kluwer Academic/Plenum Publishers, (2003), pp 405-420.
  • Durairaj, A.; Limbach, P.A. “MALDI-MS Screening for Pseudouridine in Mixtures of Small RNAs by Chemical Derivatization, RNase Digestion and Signature Products” Rapid Communications in Mass Spectrometry 22 (2008) 3727-3734. PMCID: PMC3009585.
  • Brock, J.E.; Pourshahian, S.; Limbach, P.A.; Janssen, G.R. “Ribosome Binding to Leaderless mRNA through Recognition of their 5’-terminal AUG”, RNA 14 (2008) 2159-2169. PMCID: PMC2553737.
  • Durairaj, A.; Limbach, P.A. “Mass Spectrometry of the Fifth Nucleoside: A Review of the Identification of Pseudouridine in Nucleic Acids”, Analytica Chimica Acta 623 (2008) 117-125. PMCID: PMC2597214.
  • Pourshahian, S.P.; Limbach, P.A. “Application of Delta Mass for the Identification of Peptide:Oligonucleotide Cross-links by Mass Spectrometry”, Journal of Mass Spectrometry 42 (2008) 1081-1088.
  • Menezes, S.; Gaston, K.W.; Krivos, K.V.; Apolinario, E.; Reich, N.; Sowers, K.; Limbach, P.A.; Perona, J.; “Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14”, Nucleic Acids Research 39 (2011) 7641-55. PMID: 21693558
  • Addepalli, B.; Limbach, P.A.;  “Mass Spectrometry-Based Quantification of Pseudouridine in RNA”, Journal of the American Society for Mass Spectrometry 22 (2011) 1363-1372. PMID: 21953190.
  • Wetzel, C.; Limbach, P.A.; “Global Identification of Transfer RNAs by Liquid Chromatography-Mass Spectrometry”, Journal of Proteomics, 75 (2012) 3450-3464.  (Invited article for Special Issue on Genome Regulation).
  • Phillips, G.; Swairjo, M.A.; Gaston, K.W.; Bailly, M.; Iwata-Reuyl, D.; Limbach, P.A.; de Crecy-Lagard, V.; “Diversity of Archaeosine Synthesis in Crenarchaeota”, ACS Chemical Biology, 7 (2012) 300-305.
  • Krivos, K.L.; Addepalli, B.; Limbach, P.A.; “Removal of 3’ Phosphate Group by Bacterial Alkaline Phosphatase Improves Sequence Coverage of RNase Digestion Products Analyzed by Collision Induced Dissociation Mass Spectrometry” Rapid Communications in Mass Spectrometry, 25 (2011) 3609-3616.
  • Catron, B.L.; Caruso, J.A.; Limbach, P.A.; “Selective Detection of Peptide-Oligonucleotide Heteroconjugates Utilizing Capillary HPLC-ICPMS”, Journal of the American Society for Mass Spectrometry. 23 (2012) 1053-1061.
  • Easter, R.N.; Barry, C.G.; Caruso, J.A.; Limbach, P.A.; “Separation and Identification of Phosphorothioate Oligonucleotides by Hydrophilic Interaction Liquid Chromatography Coupled to Electrospray Ionization Mass Spectrometry”, Analytical Methods 5 (2013) 2657-2659.
  • Nayak, N.; Putnam, A.; Chen, T.; Schäfermeyer, K.R.; Addepalli, B.; Lowenson, J.; Patra, B.; Jankowsky, E.; Perry, S.E.; Dinkins, R.;  Limbach, P.A.; Ray, A.; Clarke, S.G.; Downie, B.; “The DEAD-box RNA helicase, PRH75, is susceptible to isoAsp formation but can be repaired and activity restored by PROTEIN ISOASPARTYL METHYLTRANSFERASE”, The Plant Cell 25 (2013) 2573-86.
  • Butterer, A.; Zorc, M.; Castleberry, C.M.; Limbach, P.A.; “Using Immobilized Enzymes to Reduce RNase Contamination in RNase Mapping of Transfer RNAs by Mass Spectrometry”, Analytical and Bioanalytical Chemistry, 402 (2012) 2701-2711.
  • Aldinger, C.A.; Leisinger, A-K; Gaston, K.W.; Limbach, P.A.; Igloi, G.L.; “The absence of A-to-I editing in the anticodon of plant cytoplasmic tRNAArgACG demands a relaxation of the wobble rules in plants”, RNA Biology 9 (2012) 1-8.
  • Li, S.; Limbach, P.A.; “Method for Comparative Analysis of Ribonucleic Acids Using Isotope Labeling and Mass Spectrometry” Analytical Chemistry 84 (2012) 8607-8613.
  • Lokits, K.E.; Limbach, P.A.; Caruso, J.A. “Interfaces for Capillary LC with ICPMS Detection: A Comparison of Nebulizers/Spray Chamber Configurations”, J. Anal. At. Spectrom. 24 (2009) 528-534
  • Frankel, L.K.; Sallans, L.; Limbach, P.A.; Bricker, T.M.; “Identification of Oxidized Amino Acid Residues in the Vincinity of the Mn4CaO5 Cluster of Photosystem II: Implications for the Identification of Oxygen Channels within the Photosystem”, Biochemistry 51 (2012) 6371-6377.
  • Guy, M.P.; Podyma, B.M.; Preston, M.A.; Shaheen, H.H.; Krivos, K.L.;Limbach, P.A.; Hopper, A.K.; Phizicky, E.M.; “Distinct Trm7 complexes that direct anticodon loop modifications are required for efficient tRNAPhe function and healthy growth in yeast”, RNA 18 (2012) 1921-1933.
  • Russell, S.P.; Limbach, P.A.; “Evaluating the Reproducibility of Quantifying Modified Nucleosides from Ribonucleic Acids by LC-UV-MS” Journal of Chromatography, B 923–924 (2013) 74-82.
  • Wong, S.Y.; Javid, B.; Addepalli, B.; Piszczek, G.; Strader, M.B.; Limbach, P.A.; Barry III, C.E. “Functional Role of Methylation of G518 of the 16S rRNA 530 loop by GidB in Mycobacterium tuberculosisAntimicrobial Agents and Chemotherapy 57 (2013) 6311-6318.
  • Li, S.; Limbach, P.A.; “Comparative Sequencing of Transfer Ribonucleic Acids by Isotope Labeling and Mass Spectrometry”, Analyst 138 (2013) 1386-1394.
  • Frankel, L.K.; Sallans, L.; Limbach, P.A.; Bricker, T.M.; “Oxidized Amino Acid Residues in the Vicinity of QA and PheoD1 of the Photosystem II Reaction Center: Putative Generation Sites of Reducing-Side Reactive Oxygen Species” PLoS One. 8 (2013) e58042.
  • Frankel, L.K.; Sallans, L.; Bellamy, H.; Goettert, J.S.; Limbach, P.A.; Bricker, T.M.; “Radiolytic Mapping of Solvent Contact Surfaces in Photosystem II of Higher Plants: Experimental Identification of Putative Water Channels within the Photosystem” Journal of Biological Chemistry 288 (2013) 23565-23572.
  • Rodriguez-Hernandez, A.; Spears, J.; Gaston, K.W.; Limbach, P.A.; Gamper, H.; Hou, Y.-M.; Agris, P.; Perona, J.J.; “Structural and mechanistic basis for enhanced translational efficiency by 2-thiouridine at the tRNA anticodon wobble position” Journal of Molecular Biology 425 (2013) 3888-3906.
  • Wetzel, C.; Limbach, P.A.; “The Global Identification of tRNA Isoacceptors by Targeted Tandem Mass Spectrometry”, Analyst 138 (2013) 6063-6072.
  • Clatterbuck Soper, S.F.; Dator, R.P.; Limbach, P.A.; Woodson, S.A.; “In vivo X-ray footprinting of pre-30S ribosomes reveals chaperone-dependent remodeling of late assembly intermediates”, Molecular Cell 52 (2013) 506-516.
  • Köhrer, C.; Mandal, D.; Gaston, K.W.; Grosjean, H.; Limbach, P.A.; RajBhandary, U.L.; “Life without tRNAIle-lysidine synthetase: Translation of the isoleucine codon AUA in Bacillus subtilis lacking the canonical tRNA2Ile“ accepted Nucleic Acids Research.

Books

  • Banoub, J.H.; Limbach, P.A. Mass Spectrometry of Nucleosides and Nucleic Acids; Taylor & Francis, Inc., 2010.

Book Chapter

  • Hossain, M.; Limbach, P.A. Comparison of Matrices in MALDI Mass Spectrometry in Electrospray and MALDI Mass Spectrometry: Fundamentals, Instrumentation, Practicalities and Biological Applications, Cole, R. ed. (2010) John Wiley & Sons, Inc. Hoboken, New Jersey 215-244.
  • Coombs, C.C., Limbach, P.A. Electrospray Ionization Mass Spectrometry for the Direct Analysis of Modified Nucleosides in Small RNAs in Mass Spectrometry of Nucleosides and Nucleic Acids Banoub, J.H.; Limbach, P.A. eds (2010) Taylor & Francis, Inc., Boca Raton, Florida, 423-429.
  • Hossain, M., Limbach, P.A. (2010) Quantitative Identification of Nucleic Acids via Signature Digestion Products Detected Using Mass Spectrometry in Mass Spectrometry of Nucleosides and Nucleic Acids Banoub, J.H.; Limbach, P.A. eds (2010) Taylor & Francis, Inc., Boca Raton, Florida, 397-421.
  • Jin, L.; Rodicio, L.M.; Limbach, P.A. Principles of Proteomics in Angiogenesis, Functional and Medicinal Foods Losso, J.N.; Shahidi, F.; Bagchi, D., Ed(s); Marcel Dekker, Inc., (2007), pp 291-314.
  • Mecomber, J.S.; Dominick, W.D.; Jin, L.; Limbach, P.A. BioMEMS Devices for Proteomics in BioMEMS: Technologies and Applications Soper, S.A., Ed; Taylor & Francis, Inc., (2007).
  • Patteson, K.; Limbach, P.A. "Nucleic Acids: DNA and RNA Seqeuence Analysis". In Encyclopedia of Mass Spectrometry Volume 3: Biological Applications Part B: Carbohydrates, Nucleic A; Caprioli, R.; Gross, M., Ed(s); Elsevier, Inc., (2005), pp 1-10.
  • Patteson, K.; Limbach, P.A. "Nucleic Acids: Base Analysis". In Encyclopedia of Mass Spectrometry Volume 3: Biological Applications Part B: Carbohydrates, Nucleic A; Caprioli, R.; Gross, M., Ed(s); Elsevier, Inc., (2005), pp 14-18.
  • Macha, S.F.; Limbach, P.A. "Analysis of Polymeric Hydrocarbon Materials by Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry". In Analytical Advances in Hydrocarbon Research; Hsu, S., Ed(s); Kluwer Academic/Plenum Publishers: New York, N.Y., (2003), pp 385-404.
  • Castleberry, C.M.; Rodicio, L.P.; Limbach, P.A. Electrospray ionization mass spectrometry of oligonucleotides in Current protocols in nucleic acid chemistry Beaucage, S.L. [et al.] eds (2008), Chapter 10, Unit 10.2.
  • Limbach, P.A.; Hossain, M.; “Matrix-Assisted Laser Desorption/Ionization” in VOLUME V. Bioanalytical Approaches: MS, NMR, Isotope Methods, Spectroscopy, Ligand-Binding, and Other Analytical Chemistry Methods Muddiman, D.C. and Cohen, L. eds Encyclopedia of Drug Metabolism and Interactions (2012) John Wiley & Sons, Inc.

In Press

  • Wetherill, Yelena B, Hess-Wilson, Janet K, Comstock, Clay E S, Shah, Supriya A, Buncher, C Ralph, Sallans, Larry, Limbach, Patrick A, Schwemberger, Sandy, Babcock, George F, & Knudsen, Karen E (2006). Bisphenol A facilitates bypass of androgen ablation therapy in prostate cancer. Molecular cancer therapeutics, 5(12), 3181-90.
  • McLachlan, Anne, Borchers, Michael, Velayutham, Prakash, Wagner, Michael, & Limbach, Patrick A (2006) Characterizing the reproducibility of a protein profiling method for the analysis of mouse bronchoalveolar lavage fluid. Journal of proteome research, 5(11), 3059-65.
  • Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2006). Unlimited-volume electrokinetic stacking injection in sweeping capillary electrophoresis using a cationic surfactant. Analytical chemistry, 78(17), 6035-42.
  • Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2006). On-line sample preconcentration by sweeping with dodecyltrimethylammonium bromide in capillary zone electrophoresis. Journal of chromatography. A, 1125(2), 263-9.
  • Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, Arias, Francisco, & Heineman, William R (2006). On-line sample preconcentration using field-amplified stacking injection in microchip capillary electrophoresis. Analytical chemistry, 78(11), 3730-7.
  • Meng, Zhaojing, & Limbach, Patrick A (2006). Mass spectrometry of RNA: linking the genome to the proteome. Briefings in functional genomics & proteomics, 5(1), 87-95.
  • Carr, Jennifer F, Hamburg, Daisy-Malloy, Gregory, Steven T, Limbach, Patrick A, & Dahlberg, Albert E (2006). Effects of streptomycin resistance mutations on posttranslational modification of ribosomal protein S12. Journal of bacteriology, 188(5), 2020-3.
  • Mecomber, Justin S, Stalcup, Apryll M, Hurd, Doug, Halsall, H Brian, Heineman, William R, Seliskar, Carl J, Wehmeyer, Kenneth R, & Limbach, Patrick A (2006). Analytical performance of polymer-based microfluidic devices fabricated by computer numerical controlled machining. Analytical chemistry, 78(3), 936-41.
  • Piruska, Aigars, Nikcevic, Irena, Lee, Se Hwan, Ahn, Chong, Heineman, William R, Limbach, Patrick A, & Seliskar, Carl J (2005). The autofluorescence of plastic materials and chips measured under laser irradiation. Lab on a chip, 5(12), 1348-54.
  • Suh, Moo-Jin, Hamburg, Daisy-Malloy, Gregory, Steven T, Dahlberg, Albert E, & Limbach, Patrick A (2005). Extending ribosomal protein identifications to unsequenced bacterial strains using matrix-assisted laser desorption/ionization mass spectrometry. Proteomics, 5(18), 4818-31.
  • Meades, Glen D, McLachlan, Anne, Sallans, Larry, Limbach, Patrick A, Frankel, Laurie K, & Bricker, Terry M (2005). Association of the 17-kDa extrinsic protein with photosystem II in higher plants. Biochemistry, 44(46), 15216-21.
  • Currie, Christa A, Heineman, William R, Halsall, H Brian, Seliskar, Carl J, Limbach, Patrick A, Arias, Francisco, & Wehmeyer, Kenneth R (2005). Estimation of pK(a) values using microchip capillary electrophoresis and indirect fluorescence detection. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 824(1-2), 201-5.
  • Tummala, Rama, Green-Church, Kari B, & Limbach, Patrick A (2005). Interactions between sodium dodecyl sulfate micelles and peptides during matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of proteolytic digests. Journal of the American Society for Mass Spectrometry, 16(9), 1438-46.
  • Tu, Jian, Halsall, H Brian, Seliskar, Carl J, Limbach, Patrick A, Arias, Francisco, Wehmeyer, Kenneth R, & Heineman, William R (2005). Estimation of logP(ow) values for neutral and basic compounds by microchip microemulsion electrokinetic chromatography with indirect fluorimetric detection (muMEEKC-IFD). Journal of pharmaceutical and biomedical analysis, 38(1), 1-7.
  • Meng, Zhaojing, & Limbach, Patrick A (2005). Quantitation of ribonucleic acids using 18O labeling and mass spectrometry. Analytical chemistry, 77(6), 1891-5.
  • Meng, Zhaojing, & Limbach, Patrick A (2005). Shotgun sequencing small oligonucleotides by nozzle-skimmer dissociation and electrospray ionization mass spectrometry. European journal of mass spectrometry (Chichester, England), 11(2), 221-9.
  • Cameron, Dale M, Gregory, Steven T, Thompson, Jill, Suh, Moo-Jin, Limbach, Patrick A, & Dahlberg, Albert E (2004). Thermus thermophilus L11 methyltransferase, PrmA, is dispensable for growth and preferentially modifies free ribosomal protein L11 prior to ribosome assembly. Journal of bacteriology, 186(17), 5819-25.
  • Meng, Zhaojing, Simmons-Willis, Tracey A, & Limbach, Patrick A (2004). The use of mass spectrometry in genomics. Biomolecular engineering, 21(1), 1-13.
  • Suh, Moo-Jin, & Limbach, Patrick A (2004). Investigation of methods suitable for the matrix-assisted laser desorption/ionization mass spectrometric analysis of proteins from ribonucleoprotein complexes. European journal of mass spectrometry (Chichester, England), 10(1), 89-99.
  • Tummala, Rama, & Limbach, Patrick A (2004). Effect of sodium dodecyl sulfate micelles on peptide mass fingerprinting by matrix-assisted laser desorption/ionization mass spectrometry. Rapid communications in mass spectrometry : RCM, 18(18), 2031-5.
  • Hassett, Daniel J, Limbach, Patrick A, Hennigan, Robert F, Klose, Karl E, Hancock, Robert E W, Platt, Mark D, & Hunt, Donald F (2003). Bacterial biofilms of importance to medicine and bioterrorism: proteomic techniques to identify novel vaccine components and drug targets. Expert opinion on biological therapy, 3(8), 1201-7.
  • Berhane, Beniam T, & Limbach, Patrick A (2003). Stable isotope labeling for matrix-assisted laser desorption/ionization mass spectrometry and post-source decay analysis of ribonucleic acids. Journal of mass spectrometry : JMS, 38(8), 872-8.
  • Dominick, Wendy D, Berhane, Beniam T, Mecomber, Justin S, & Limbach, Patrick A (2003). Covalent immobilization of proteases and nucleases to poly(methylmethacrylate). Analytical and bioanalytical chemistry, 376(3), 349-54.
  • Berhane, Beniam T, & Limbach, Patrick A (2003). Functional microfabricated sample targets for matrix-assisted laser desorption/ionization mass spectrometry analysis of ribonucleic acids. Analytical chemistry, 75(9), 1997-2003.
  • Robins, Chad, & Limbach, Patrick A (2003). The use of nonpolar matrices for matrix-assisted laser desorption/ionization mass spectrometric analysis of high boiling crude oil fractions. Rapid communications in mass spectrometry : RCM, 17(24), 2839-45.
  • Chou, Chau-Wen, Limbach, Patrick A, & Cole, Richard B (2002). Fragmentation pathway studies of oligonucleotides in matrix-assisted laser desorption/ionization mass spectrometry by charge tagging and H/D exchange. Journal of the American Society for Mass Spectrometry, 13(12), 1407-17.
  • Limbach, Patrick A, & Meng, Zhaojing (2002). Integrating micromachined devices with modern mass spectrometry. The Analyst, 127(6), 693-700.
  • Macha, S F, Limbach, P A, Hanton, S D, & Owens, K G (2001). Silver cluster interferences in matrix-assisted laser desorption/ionization (MALDI) mass spectrometry of nonpolar polymers. Journal of the American Society for Mass Spectrometry, 12(6), 732-43.
  • Patteson, K G, Rodicio, L P, & Limbach, P A (2001). Identification of the mass-silent post-transcriptionally modified nucleoside pseudouridine in RNA by matrix-assisted laser desorption/ionization mass spectrometry. Nucleic acids research, 29(10), E49-9.
  • Polo, L M, & Limbach, P A (2001). Analysis of oligonucleotides by electrospray ionization mass spectrometry. Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage. [et al.], Chapter 10, Unit 10.2.
  • Chou, C W, & Limbach, P A (2001). Analysis of oligonucleotides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage. [et al.], Chapter 10, Unit 10.1.
  • Meng, Z, Qi, S, Soper, S A, & Limbach, P A (2001). Interfacing a polymer-based micromachined device to a nanoelectrospray ionization Fourier transform ion cyclotron resonance mass spectrometer. Analytical chemistry, 73(6), 1286-91.
  • Green-Church, K B, Limbach, P A, Freitas, M A, & Marshall, A G (2001). Gas-phase hydrogen/deuterium exchange of positively charged mononucleotides by use of Fourier-transform ion cyclotron resonance mass spectrometry. Journal of the American Society for Mass Spectrometry, 12(3), 268-77.
  • Macha, Limbach, & Savickas, (2000). Application of nonpolar matrices for the analysis of low molecular weight nonpolar synthetic polymers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Journal of the American Society for Mass Spectrometry, 11(8), 731-7.
  • Ragas, J A, Simmons, T A, & Limbach, P A (2000). A comparative study on methods of optimal sample preparation for the analysis of oligonucleotides by matrix-assisted laser desorption/ionization mass spectrometry. The Analyst, 125(4), 575-81.
  • Breaux, G A, Green-Church, K B, France, A, & Limbach, P A (2000). Surfactant-aided, matrix-assisted laser desorption/ionization mass spectrometry of hydrophobic and hydrophilic peptides. Analytical chemistry, 72(6), 1169-74.
  • Green-Church, K B, & Limbach, P A (2000). Mononucleotide gas-phase proton affinities as determined by the kinetic method. Journal of the American Society for Mass Spectrometry, 11(1), 24-32.
  • Vandell, V E, & Limbach, P A (1999). Polyamine co-matrices for matrix-assisted laser desorption/ionization mass spectrometry of oligonucleotides. Rapid communications in mass spectrometry : RCM, 13(20), 2014-21.
  • Polo, L M, & Limbach, P A (1998). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for the analysis of RNase H cleavage products. Journal of mass spectrometry : JMS, 33(12), 1226-31.
  • Vandell, V E, & Limbach, P A (1998). Electrospray ionization mass spectrometry of metalloporphyrins. Journal of mass spectrometry : JMS, 33(3), 212-20.
  • Mangroo, D, Limbach, P A, McCloskey, J A, & RajBhandary, U L (1995). An anticodon sequence mutant of Escherichia coli initiator tRNA: possible importance of a newly acquired base modification next to the anticodon on its activity in initiation. Journal of bacteriology, 177(10), 2858-62.
  • Limbach, P A, Crain, P F, & McCloskey, J A (1995). Characterization of oligonucleotides and nucleic acids by mass spectrometry. Current opinion in biotechnology, 6(1), 96-102.
  • Limbach, P A, Crain, P F, Pomerantz, S C, & McCloskey, J A (1995). Structures of posttranscriptionally modified nucleosides from RNA. Biochimie, 77(1-2), 135-8.
  • Limbach, P A, Crain, P F, & McCloskey, J A (1994). Summary: the modified nucleosides of RNA. Nucleic acids research, 22(12), 2183-96.
  • Castleberry, Colette M, Chou, Chau-Wen, & Limbach, Patrick A (2008). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligonucleotides. Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage. [et al.], Chapter 10, Unit 10.1.
  • Durairaj, Anita, & Limbach, Patrick A (2008). Improving CMC-derivatization of pseudouridine in RNA for mass spectrometric detection. Analytica chimica acta, 612(2), 173-81.
  • Gong, Maojun, Nikcevic, Irena, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2008). Protein-aptamer binding studies using microchip affinity capillary electrophoresis. Electrophoresis, 29(7), 1415-22.
  • Platt, Mark D, Schurr, Michael J, Sauer, Karin, Vazquez, Gustavo, Kukavica-Ibrulj, Irena, Potvin, Eric, Levesque, Roger C, Fedynak, Amber, Brinkman, Fiona S L, Schurr, Jill, Hwang, Sung-Hei, Lau, Gee W, Limbach, Patrick A, Rowe, John J, Lieberman, Michael A, Barraud, Nicolas, Webb, Jeremy, Kjelleberg, Staffan, Hunt, Donald F, & Hassett, Daniel J (2008). Proteomic, microarray, and signature-tagged mutagenesis analyses of anaerobic Pseudomonas aeruginosa at pH 6.5, likely representing chronic, late-stage cystic fibrosis airway conditions. Journal of bacteriology, 190(8), 2739-58.
  • Mecomber, Justin S, Murthy, Rajesh S, Rajam, Sridhar, Singh, Pradeep N D, Gudmundsdottir, Anna D, & Limbach, Patrick A (2008). Photochemical functionalization of polymer surfaces for microfabricated devices. Langmuir : the ACS journal of surfaces and colloids, 24(7), 3645-53.
  • Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2007). Flow manipulation for sweeping with a cationic surfactant in microchip capillary electrophoresis. Journal of chromatography. A, 1167(2), 217-24.
  • Duderstadt, Randall E, Tsuie, Barbara M, Macha, Stephen F, & Limbach, Patrick A (2007). Investigation of single-site zirconium azaborolinyl complexes by laser desorption ionization time of flight mass spectrometry. Analytica chimica acta, 596(1), 124-31.
  • Suh, Moo-Jin, Pourshahian, Soheil, & Limbach, Patrick A (2007). Developing limited proteolysis and mass spectrometry for the characterization of ribosome topography. Journal of the American Society for Mass Spectrometry, 18(7), 1304-17.
  • Nikcevic, Irena, Lee, Se Hwan, Piruska, Aigars, Ahn, Chong H, Ridgway, Thomas H, Limbach, Patrick A, Wehmeyer, K R, Heineman, William R, & Seliskar, Carl J (2007). Characterization and performance of injection molded poly(methylmethacrylate) microchips for capillary electrophoresis. Journal of chromatography. A, 1154(1-2), 444-53.
  • Gong, Maojun, Wehmeyer, Kenneth R, Stalcup, Apryll M, Limbach, Patrick A, & Heineman, William R (2007). Study of injection bias in a simple hydrodynamic injection in microchip CE. Electrophoresis, 28(10), 1564-71.
  • Gong, Maojun, Wehmeyer, Kenneth R, Limbach, Patrick A, & Heineman, William R (2007). Frontal analysis in microchip CE: a simple and accurate method for determination of protein-DNA dissociation constant. Electrophoresis, 28(5), 837-42.
  • Hossain, Mahmud, & Limbach, Patrick A (2007). Mass spectrometry-based detection of transfer RNAs by their signature endonuclease digestion products. RNA (New York, N.Y.), 13(2), 295-303.

Prefaces

  • Lubman, D.M.; Limbach, P.A. "Mass spectrometry. Preface." Analytica Chimica Acta, 627(1), (2008) 1-2.

Review

  • Fabris, D, & Limbach, P A (2010). Review of the 22nd Sanibel Conference on mass spectrometry: from structural biology to drug discovery: new roles for mass spectrometry of nucleic acids. Journal of the American Society for Mass Spectrometry, 21(7), R1-4.

Presentations & Lectures

Invited Presentations

  • (11-2007). Modified RNAs: From Cancer to Biochemistry Bethany College, Department of Chemistry.
  • (03-2007). Dissecting RNAs and RNPs in the Gas Phase Brown University, Department of Microbiology and Biochemistry.
  • (01-2007). Mass Spectrometry as a Tool to Study RNA and RNA:Protein Complexes Rennselaer Polytechnic Institute, Department of Chemistry.
  • (11-2006). Optimizing Surface Chemistries to Develop Plastic Microfluidic and Microarray Devices for Mass Spectrometry Eastern Analytical Symposium.
  • (11-2005). RNA for the Masses University of Cincinnati, Department of Biological Sciences.
  • (06-2005). Developing Mass Spectrometry Approaches for RNA and RNP Complexes Ohio State University, Department of Molecular Virology, Immunology and Human Genetics.
  • (04-2005). Protein Factories on Bathroom Scales John Carroll University, Department of Chemistry.
  • (11-2004). Microchip Mass Spectrometry: Plastics Make it Possible Bradley University, Department of Chemistry.
  • (06-2004). Optimizing Surface Chemistries to Develop Plastic Microfluidic and Microarray Devices for Mass Spectrometry 87th Canadian Chemistry Conference and Exhibition.
  • (06-2004). Mass Spectrometry as a Tool for Probing Ribosome Structure trometry as a Tool for Probing Ribosome Structure”, .
  • (04-2004). Profiling the Source of Proteins University of Oregon, Institute of Molecular Biology.
  • (04-2004). Developing Mass Spectrometry Approaches for RNP Complexes University of California, Riverside, Department of Chemistry.
  • (11-2003). RNA for the Masses: Using Mass Spectrometry to Characterize RNA Structure 2003 Rustbelt RNA Meeting.
  • (11-2003). Interfacing Microchips to Mass Spectrometry NSF Workshop on Miniaturization and Mass Spectrometry.
  • (10-2003). Microchip Mass Spectrometry: Plastics Make it Possible University of Maryland, Baltimore County, Department of Chemistry.
  • (10-2003). Profiling the Source of Proteins: Mass Spectrometry of the Ribosome 35th ACS Central Regional Meeting.
  • (09-2003). Bioassays and Molecular Characterization by Microchip CE and Microchip MS 226th ACS National Meeting.
  • (02-2003). Integrating Microfabricated Devices with Modern Mass Spectrometry LabAutomation, Palm Springs, CA.
  • (01-2010). Quantitative Identification of Modified Nucleic Acids ASMS Sanibel Conference, St. Pete Beach, FL.
  • (04-2010). Characterizing Modified Nucleic Acids Using Mass Spectrometry Department of Chemistry Highlands Seminar Series, Virginia Tech University.
  • (05-2010). Characterizing Modified Nucleic Acids Using Mass Spectrometry Department of Chemistry, Queens Univ Kingston Ontario CANADA.
  • (01-2011). RNA Spell Checking: Approaches for Identifying Editing and Modification Sites Gordon-Kenan Research Seminar on Editing and Modification of RNA and DNA, Galveston Island Texas.
  • (01-2011). Advanced Mass Spectrometry Studies of RNA Modification Gordon Research Conference on RNA Editing and Modification, Galveston Island Texas.
  • (02-2011). What's Wrong with Plain old RNA? Department of Chemistry, Northern Kentucky University.
  • (02-2011). Fundamentals of Bioanalytical Mass Spectrometry Department of Chemistry, Virginia Tech University.
  • (04-2011). The Utility of Mass Spectrometry for Studying RNA Modification and Editing Frontiers in Proteomics, Univ Massachusetts Medical School, Worcester MA.
  • (05-2011). Ion Mobility Mass Spectrometry for Oligonucleotide Impurity Profiling Nucleic Acids Technologies for Diagnostics: Analytical Methods and Validation, TIDES 2011, Boston MA.
  • (11-2011). What's Wrong with Plain old RNA? Department of Chemistry, Wittenberg University.
  • (04-2012). Using Mass Spectrometry to Study RNA Modification Department of Chemistry, Wayne State University.
  • (05-2012). Analytical Characterization of Branched Oligonucleotides TIDES 2012 Meeting, Las Vegas, NV.
  • (05-2012). New Mass Spectrometry Assays for Identifying and Characterizing Modified RNAs 60th ASMS conference on Mass Spectrometry and Allied Topics, Vancouver, Canada.
  • (01-2012). What’s Wrong with Plain Old RNA: Identifying Modified RNAs and Understanding their Health Significance University of Louisville Department of Chemistry.
  • (10-2012). RNA Spell-Checking: How to Know when RNA is Modified and Why Indiana State University Department of Chemistry.
  • (08-2012). Mass Spectrometry for the Identification of Natural and Synthetic Oligonucleotides Food and Drug Administration, Cincinnati, OH.
  • (02-2013). RNA Spell-Checking: How to Know when RNA is Modified and Why Department of Chemistry, Mt. St. Joseph College.
  • (02-2013). Studying RNA Editing and Modification Using Mass Spectrometry Chemical Biology Program, University of Minnesota.
  • (04-2013). Mass Spectrometry for the Analysis of Modified RNAs and RNA-Protein Cross-links RNA Center Speaker Series, The Ohio State University.
  • (10-2013). High Performance Mass Spectrometry for Structural and Conformational Analysis of Natural and Therapeutic Oligonucleotides Waters Corporation, Webinar Series.

Paper Presentations

  • M. Hossain, P.A. Limbach (10-2007). Large Scale Quantitive Analysis of Non-coding RNAs (ncRNAs) by their Signature Digestion Products using Stable Isotope Labeling and MALDI-MS. of Analytical Chemistry and Spectroscopy Societies (FACSS), Memphis, TN.
  • Kirk E. Lokits, Scott Afton, Karolin Kroening, Renee Kepler, Patrick A. Limbach and Joseph A. Caruso (10-2007). ICPMS Collision Cell: Novel Applications with Xenon Gas. Federation of Analytical Chemistry and Spectroscopy Societies (FACSS), Memphis, TN.
  • C.M. Castleberry, L. Sallans, P.A. Limbach (05-2007). Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.
  • M. Hossain, P.A. Limbach (05-2007). Relative Quantification of Large Ribosomal RNAs (rRNAs) by their Signature Digestion Products. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.
  • L. Sallans, Y.B. Wetherill, J.K. Hess-Wilson, C.E.S. Comstock, S.A. Shah, C.R. Buncher, P.A. Limbach, S. Schwemberger, G.F. Babcock, and K.E. Knudsen (05-2007). Bisphenol A Facilities Bypass of Androgen Ablation Therapy in Prostrate cancer: Use of Mass Spectrometry to determine Bisphenol A Levelsin the Blood Serum of Mice. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.
  • C.M. Castleberry, L. Sallans, P.A. Limbach (03-2007). Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS. Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH .
  • D.-M Hamburg, M.-J. Suh and P.A. Limbach (03-2007). Conformational Changes and Chemical Reactivity of the Ribosome: An application of Limited Proteolysis and Matrix-assisted Laser Desorption/Ionizatization Mass Spectrometry. ”, Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH.
  • M. Hossain, P.A. Limbach (03-2007). Playing SUDOKU with Nucleotides. Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH.
  • R.L. Rohlfs, P.A. Limbach (03-2007). Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotic. Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH.
  • D.-M Hamburg, M.-J Suh and P.A. Limbach (02-2007). Mass Spectrometry of Ribosomes for structural Elucidation. Pittcon, Chicago, IL.
  • A.Durairaj, P.A. Limbach (03-2006). Optimizing a Mass Spectrometry-Based Approach for the Detection of CMC-derivatized Pseudouridine in RNA. Third Annual Mass spectrometry Symposium, Quest Conference Center, Columbus, OH.
  • C.M. Castleberry, L.Sallans, P. A. Limbach (03-2006). Developing an L.C.-MS Approach for Quantifying RNAs. Third Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH.
  • M. Hossain, P.A. Limbach (03-2006). Maximizing the Detection of transfer RNAs by Multiple Ribonucleases. Third Annual Ohio Mass Spectrometry Consortium Symposium, Quest Conference Center, Columbus, OH .
  • A.Piruska, I. Nikcevic, P. A. Limbach, W. R. Heineman, C. J. Seliskar (01-2006). Optical Detection System for Multi-Lane Plastic Microchips. 10th LabAutomation 2006, Palm Springs, CA.
  • A.L. Dupre, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P. A. Limbach (11-2005). Polymer Microchips for Capillary Electrophoresis Electrospray Ionization Mass Spectrometyr (CE-ESI-MS). American Institute for Chemical Engineers National Meeting, Cincinnati, OH.
  • D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (11-2005). Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. American Institute for Chemical Engineers National Meeting, Cincinnati, OH.
  • D.-M. Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg (10-2005). Analysis of Streptomycin Resistant and Dependent Thermus Thermophilus Ribosomal Protein S12 Mutants via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. 2005 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • M. Hossain, P.A. Limbach (10-2005). In Search of Signature Digestion Products: tRNA Treasure Hunting. 2005 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • A.Piruska, I. Nikcevic, S.H. Lee, C.H. Ahn, P.A. Limbach, W.R. Heineman, C.J. Seliskar (08-2005). Laser Induced Fluorescence Detection System for Multi-Lane Plastic Microchips. 230th ACS Meeting, Washington, DC.
  • C. Coombs, P.A. Limbach (07-2005). Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs. Montreux LC/MS Symposium, Ithaca, NY.
  • M. Hossain, P.A. Limbach (05-2005). Ribonuclease T1 Mediated Cleavage and MALDI-MS for Detection and Quantification of Escherichia coli tRNAs. American Society for Mass Spectrometry, San Antonio, TX.
  • A.L. Dupre, W.D. Dominick, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach (03-2005). EOF Characterization of Surface-Modified PMMA Microchips for Use With Electrospray Ionization Mass Spectrometry. Ohio Mass Spectrometry Symposium, The Ohio State University, Columbus, OH .
  • D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (03-2005). Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Second Annual Mass Spectrometry Symposium, Ohio State University, Columbus, OH.
  • M. Hossain, P.A. Limbach (03-2005). Detection and Quantification of Escherichia coli tRNAs by MALDI-MS. Second Annual Mass Spectrometry Symposium, Ohio State University, Columbus, OH.
  • C. Coombs, P.A. Limbach (03-2005). Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs. Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.
  • M.J. Suh, S. Pourshahian, P.A. Limbach (05-2004). Probing the Stability of Ribonucleoprotein Complexes by Mass Spectrometry. 52nd ASMS Conference on Mass Spectrometry and Allied Topics.
  • W.D. Dominick, A. Dupre, L. Jin, J. Mecomber, P.A. Limbach (05-2004). Plastic Microchips for Mass Spectrometry. 52nd ASMS Conference on Mass Spectrometry and Allied Topics.
  • A. Durairaj, K.G. Patteson, P.A. Limbach (03-2004). Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA. First Annual Ohio Mass Spectrometry Symposium.
  • Z. Meng, P.A. Limbach (03-2004). RNase Mapping of Intact Nucleic Acids by ESI-FTICRMS and 18O Labeling. First Annual Ohio Mass Spectrometry Symposium.
  • R. Tummala, K.B. Green-Church, P.A. Limbach (03-2004). Effect of Sodium Dodecyl Sulfate on MALDI-MS Analysis of Proteolytic Digests. First Annual Ohio Mass Spectrometry Symposium.
  • M.-J. Suh, P.A. Limbach (03-2004). Investigation of Methods Suitable for the MALDI Mass Spectrometric Analysis of Proteins from Ribonucleoprotein Complexes. First Annual Ohio Mass Spectrometry Symposium.
  • M.J. Suh, S. Pourshahian, P.A. Limbach (10-2003). Developing Cross-Linking and MALDI-TOFMS Approaches for Exploring Ribosomal Assembly and Interactions. 2003 ASMS Asilomar Conference.
  • B.T. Berhane, P.A. Limbach (06-2003). Functional Microfabricated Devices for the Mass Spectral Analysis of Nucleic Acids. 51st ASMS Conference on Mass Spectrometry and Allied Topics.
  • S.N. Mounicou, J. Meija, P.A. Limbach, J.A. Caruso (06-2003). The Synergy of Tandem Atomic and Molecular Mass Spectrometry Approaches for Characterizing Metalloproteins. 51st ASMS Conference on Mass Spectrometry and Allied Topics.
  • C. Robins, P.A. Limbach (03-2003). Adding Selectivity to MALDI-MS for Nonpolar Analytes. The 30th Annual Conference of NOBCChE.
  • Collin Wetzel and Patrick A. Limbach (04-2012). High throughput global identification of tRNA through the targeted detection of unique transitions. 9th Annual Ohio Mass Spectrometry Symposium, Columbus, OH.
  • Collin Wetzel and Patrick A. Limbach (10-2012). Targeted Tandem Mass Spectrometry for tRNA Identification. Rustbelt RNA Meeting, Dayton, OH.
  • Wunan Shi, Collin Wetzel and Patrick A. Limbach (04-2012). High throughput De Novo Sequencing of Total tRNAs in Thermus thermophulis Using LC-MS/MS. 9th Annual Ohio Mass Spectrometry Symposium, Columbus, OH.
  • Xiaoyu Cao and Patrick A. Limbach (04-2013). Inosine at the Wobble Position of tRNAArg in Thermus thermophilus. 10th Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Yang Jiao and Patrick A. Limbach (04-2013). Ligating oligonucleotide and NAD+ with T4 RNA ligase. 10th Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Robert Ross and Patrick A. Limbach (04-2013). Thermus thermophilus tRNAs Asn, Asp, His, and Tyr contain guanosine rather than queuosine at position 34. 10th Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Collin Wetzel and Patrick A. Limbach (04-2013). Automated data mining for C13 depleted RNA. 10th Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Collin Wetzel and Patrick A. Limbach (06-2013). Targeted Tandem Mass Spectrometry for tRNA Identification. 61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN.
  • Sarah F.C. Soper, Hajin Kim, Sanjaya Abeysirigunawardena, Romel Dator, Taekjip Ha, Patrick Limbach, Megan Mayerle, Kaushik Ragunathan, Sarah Woodson (07-2013). Visualizing RNA Folding and Dynamics During Ribosome Assembly in Bacteria. Ribosomes Conference, Napa Valley, CA.
  • B. Tekaligne, B. Addepalli, P. A. Limbach (08-2013). Role of rRNA domains in bacterial ribosome subunit assembly. University of Cincinnati’s Women in Science and Engineering Program (WISE), Cincinnati, OH.

Poster Presentations

  • M. Hossain, P.A. Limbach (10-2007). Large Scale Quantitive Analysis of Non-coding RNA (ncRNAs) by their Signature Digestion Products using Stable Isotope Labeling and MALDI-MS NIH 2nd NGSRF 2007, Bethesda, MD.
  • C.M Castleberry, L.Sallans, and P.A. Limbach (06-2007). Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.
  • D.-M Hamburg, M.-Suh and P.A. Limbach (06-2007). Conformational Changes and Chemical Reactivity of the Ribosome: An Application of Limited Proteolysis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.
  • M. Hossain, P.A. Limbach (06-2007). Global Quantification of tRNAs by their Signature Digestion Products and MALDI-MS 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.
  • Kady L. Krivos, Larry Sallans, Patrick A. Limbach (06-2007). Comparing Collision Induced Dissociation and Electron Capture Dissociation for the Sequencing of Peptide:Oligonucleotide Heteroconjugate 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.
  • S. Pourshahian, P.A. Limbach (06-2007). Identification of Oligonucleotide-Peptide cross-links using mass defect ", 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.
  • R.L. Rohlfs, P.A. Limbach (06-2007). Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the Presence of Antibiotics 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.
  • Kady L. Krivos, Larry Sallans, Patrick A. Limbach (05-2007). Comparison of Collision Induced Dissociation and Electron Capture Dissociation for the Sequencing of Peptide:Oligonucleotide Heteroconjugate Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.
  • R.L. Rohlfs, P.A. Limbach (05-2007). Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotics Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.
  • S. Russell, P.A. Limbach (05-2007). Posttranscriptional Modifications in Bacterial rRNA and Heat Shock Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.
  • C.M. Castleberry, L. Sallans, P.A. Limbach (03-2007). Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.
  • M. Hossain, P.A. Limbach (03-2007). Quantification of Large Ribosomal RNAs (rRNAs) in Escherichia coli Graduate Poster Forum, University of Cincinnati.
  • Kady L. Krivos, Larry Sallans, Patrick A. Limbach (03-2007). Implementing Electron Capture Dissociation as a Method of the Assessment of Cross Linked Protein-Nucleic Acid Complexes Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.
  • R.L. Rohlfs, P.A. Limbach (03-2007). Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotic Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.
  • Kady L. Krivos, Larry Sallans, Patrick A. Limbach (03-2007). Comparing Collision Induced Dissociation and Electron Capture dissociation for the Assessment of RNA-Peptide Complexes Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH.
  • D.-M Hamburg, D.D. Richardson, P.A. Limbach and J.A. Caruso (02-2007). Investigation of Metals in Ribosomal Proteins European Winter Plasma Conference, Taormino, Italy.
  • M. Hossain, C. Cherenfant, P.A. Limbach (02-2007). Detection of Cytosolic and Mitochondrial Transfer RNAs of Saccharomyces by their Signature Digestion Products Pittcon, Chicago, IL.
  • S. Pourshahian, P.A. Limbach (02-2007). Application of Mass Defect to Identification of Oligonucleotide-Peptide Cross-links Pittcon, Chicago, IL.
  • A.Durairaj, P.A. Limbach (10-2006). Identifying Pseudouridines in RNA by Mass Spectrometry 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • C.M. Castleberry L. Sallans, P.A. Limbach (10-2006). Quantitation of Isotopically Labeled RNA Using LC-MS 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • D.-M Hamburg, M.-J. Suh and P.A. Limbach (10-2006). Probing the Topography Ribonucleoprotein Complexes Using Limited Proteolysis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • M. Hossain, P.A. Limbach (10-2006). Detection of Mitochondrial Transfer RNAs Import of Saccharomyces Cerevisiae by their Signature Digestion Products 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • S. Russell, P.A. Limbach (10-2006). Posttranscriptional Modifications in Bacterial rRNA and Heat Shock 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • C.M. Castleberry, L. Sallans, P.A. Limbach (10-2006). Quantitation of Isotopically Labeled RNA Using LC-MS Oesper Symposium, University of Cincinnati, Cincinnati, OH.
  • D.-M Hamburg, M.-J. Suh and P.A. Limbach (10-2006). Probing the Topography Ribonucleoprotein Complexes Using Limited Proteolysis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Oesper Symposium, University of Cincinnati, Cincinnati, OH.
  • Kady L. Krivos, Larry Sallans, Patric A. Limbach (10-2006). Implementing Electron Capture Dissociation as a Method of the Assessment of Cross Linked Protein-Nucleic Acid Complexes Oesper Symposium, University of Cincinnati, Cincinnati, OH.
  • R.L. Rolfs, P.A. Limbach (10-2006). Posttranslational and Posttranscriptional Modifications to the Large Ribosomal Subunit of Escherichia coli Grown in the Presence of Antibiotic Oesper Symposium, University of Cincinnati, Cincinnati, OH.
  • M. Hossain, P.A. Limbach (10-2006). Detection of Mitochondrial Transfer RNAs Import of Saccharomyces Cerevisiae by their Signature Digestion Products 2006 Oesper Symposium, University of Cincinnati.
  • A.Durairaj, P.A. Limbach (06-2006). Optimizing a Mass Spectrometry-Based Approach for the Detection of CMC-derivatized Pseudouridine in RNA 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.
  • D.-M Hamburg, M.-J. Suh and P.A. Limbach (05-2006). Limited Proteolysis and Matrix-assisted Laser Desorption/Ionization Mass Spectrometry for the Interpretation of Ribosomal Subunit Topography 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.
  • M. Hossain, P.A. Limbach (05-2006). Signature Digestion Product of Transfer RNA’s Advantages of Multiple Ribonucleases 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.
  • S. Macha, S. Russell, L. Sallans, and P.A. Limbach (05-2006). Evaluation of In-gel Protein Digestion protocols: How Important are the Steps Involved? 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.
  • S. Pourishahian, P.A. Limbach (05-2006). Characterization of Ribosomes by Chemical Cross-linking and Mass Spectrometry 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.
  • C.M Castleberry, P.A. Limbach (03-2006). Developing an LC-MS Approach for Quantifying RNAs University of Cincinnati Graduate Poster Forum, Cincinnati, OH.
  • D.-M Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg (03-2006). Adventure Ribosomes Using Matrix-Assisted Laser desorption/Ionization Time-of-Flight Spectometry University of Cincinnati Graduate Poster Forum, Cincinnati, OH.
  • M. Hossain P.A. Limbach (03-2006). Augmenting Transfer Ribonucleic Acids (tRNAs) Escherichia coli by using Multiple Endoribonucleases University of Cincinnati Graduate Poster Forum, Cincinnati, OH.
  • D.-M Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg (03-2006). Adventures with Ribosomes Using Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry American Chemical Society National Meeting, Atlanta, GA.
  • I.Nikcevic, S-H. Lee, A. Piruska, C. H. Ahn, P.A. Limbach, W. R. Heineman, C. J. Seliskar (01-2006). Characterization of Cyclic Olefin Copolymer (COC) and Poly(methylmethacrylate) (PMMA) Microchips for Capillary Electrophoresis 10th LabAutomation 2006, Palm Springs, CA.
  • C. Coombs, P.A. Limbach (10-2005). Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • A.L. Dupre, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach (10-2005). Polymer Microchips for Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS) Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (10-2005). Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • M. Hossain, P.A. Limbach (10-2005). On the Heels of Signature Digestion Products: tRNA Treasure Hunting Oesper Symposium, University of Cincinnati.
  • S. Pourshahian, M.-J. Suh, P.A. Limbach (10-2005). Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry Oesper Symposium, University of Cincinnati.
  • C. M. Castleberry, P.A. Limbach (10-2005). Developing an LC-MS Approach for Quantifying RNAs 2005 Rustbelt RNA Meeting, Deer Creek State Park & Resort, Mt. Sterling, OH.
  • C. M. Castleberry, P.A. Limbach (10-2005). Developing an LC-MS Approach for Quantifying RNAs Oesper Symposium, University of Cincinnati, Cincinnati, OH.
  • C. Coombs, P.A. Limbach (10-2005). Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs 2005 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • A.Durairaj, P.A. Limbach (10-2005). Mapping Pseudouridines in RNA by MALDI Spectrometry 2005 Rustbelt RNA Meeting, Mt. Sterling, OH.
  • C. Coombs, P.A. Limbach (05-2005). Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs American Society for Mass Spectrometry, San Antonio, TX.
  • D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (05-2005). Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry American Society for Mass Spectrometry, San Antonio, TX.
  • S. Pourshahian, M.-J. Suh, P.A. Limbach (05-2005). Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry American Society for Mass Spectrometry, San Antonio, TX.
  • A.L. Dupre, W.D. Dominick, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach (03-2005). EOF Characterization of Surface-Modified PMMA Microchips for Use With Electrospray Ionization Mass Spectrometry Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.
  • M. Hossain, P.A. Limbach (03-2005). A Facile Method for Detection and Quantification of Escherichia coli tRNAs by MALDI-MS Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.
  • A. Durairaj, K.G. Patteson, P.A. Limbach (11-2004). Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA 2004 Rustbelt RNA Meeting.
  • S. Pourshahian, M.-J. Suh, P.A. Limbach (11-2004). Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry 2004 Rustbelt RNA Meeting.
  • P.A. Limbach, M. Hossain, Z. Meng, S. Pourshahian, M.J. Suh (06-2004). Mass Spectrometry as a Tool for Probing Ribosome Structure Ninth Annual Meeting of the RNA Society.
  • A. Dupre, W.D. Dominick, J. Mecomber, H.B. Halsall, W.R. Heineman, C.J. Seliskar, K.R. Wehmeyer, P.A. Limbach (05-2004). EOF Characterization of Surface-Modified PMMA Microchips ALA Lab Fusion.
  • C. Robins, P.A. Limbach (05-2004). Initial Ion Velocities During Charge-Transfer Ionization in MALDI-MS 52nd ASMS Conference on Mass Spectrometry and Allied Topics.
  • A. Durairaj, P.A. Limbach (05-2004). Towards Affinity Purification and Mass Spectrometric Analysis of Pseudouridines in RNA 52nd ASMS Conference on Mass Spectrometry and Allied Topics.
  • Z, Meng, M. Hossain, P.A. Limbach (05-2004). Modification Mapping, Sequencing and Quantitation of Nucleic Acids 52nd ASMS Conference on Mass Spectrometry and Allied Topics.
  • M. Tummala, L.K. Frankel, T.M. Bricker, P.A. Limbach (05-2004). Determining Protein-Protein Interactions in the Oxygen-evolving Complex (OEC) of Photosystem II by Mass Spectrometry 52nd ASMS Conference on Mass Spectrometry and Allied Topics.
  • S.B. Jayasinghe, S. Mounicou, T.D. Grant, P.A. Limbach, J.A. Caruso (05-2004). Investigation of Metalloproteins in Soy Bean Proteome by Chromatographic and Mass Spectrometric Techniques 52nd ASMS Conference on Mass Spectrometry and Allied Topics.
  • L. Jin, M. Sperling, A. Pesce, M. Wagner, P.A. Limbach (05-2004). Exploring MALDI-MS for Biomarker Screening of Colon Cancer 52nd ASMS Conference on Mass Spectrometry and Allied Topics.
  • J. Mecomber, D. Hurd, P.A. Limbach (02-2004). Rapid and Cost-Effective Prototyping of Plastic Microfabricated Devices for Mass Spectrometry Lab Automation 2004.
  • A.Durairaj, K.G. Patteson, P.A. Limbach (11-2003). Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA 2003 Rustbelt RNA Meeting.
  • A.Durairaj, K.G. Patteson, P.A. Limbach (11-2003). Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA 2003 Rustbelt RNA Meeting.
  • M.J. Suh, S. Pourshahian, P.A. Limbach (11-2003). Developing Cross-Links and MALDI-TOFMS Approaches for Exploring Ribosomal Assembly and Interactions 2003 Rustbelt RNA Meeting.
  • P.A. Limbach, K.G. Patteson, A. Durairaj (06-2003). Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNAs 51st ASMS Conference on Mass Spectrometry and Allied Topics.
  • Z. Meng, P.A. Limbach (06-2003). Shotgun Sequencing of Modified RNAs by Nozzle-Skimmer ESI-MS 51st ASMS Conference on Mass Spectrometry and Allied Topics.
  • J.S. Mecomber, P.A. Limbach (06-2003). Rapid and Cost-effective Prototyping of Plastic Microfabricated Devices for Mass Spectrometry 51st ASMS Conference on Mass Spectrometry and Allied Topics.
  • R. Tummala, K.B. Green-Church, P.A. Limbach (05-2003). Effect of Sodium Dodecyl Sulfate on MALDI-MS Analysis of Proteolytic Digests ”, 51st ASMS Conference on Mass Spectrometry and Allied Topics.
  • M.-J. Suh, P.A. Limbach (05-2003). Developing a MALDI Mass Spectrometry Approach for Probing Ribosomal Assembly and Interactions 51st ASMS Conference on Mass Spectrometry and Allied Topics.
  • W.D. Dominick, P.A. Limbach (05-2003). Polymethylmethacrylate (PMMA) Microfabricated Devices for Mass Spectrometry 51st ASMS Conf. on Mass Spectrometry and Allied Topics.
  • W.D. Dominick, P.A. Limbach (02-2003). Surface Modification of Polymethylmethacrylate (PMMA) Microfabricated Devices for Improved Protein Characterization Lab Automation.
  • Kady L. Krivos, Larry Sallans, Patrick A. Limbach (03-2008). “Investigation of electron capture dissociation (ECD) as an alternative to collision induced dissociation (CID) for the sequencing of peptide:oligonucleotide heteroconjugates” Pittcon 2008, New Orleans, LA.
  • C.M. Castleberry, L. Sallans, R. Kepler, P.A. Limbach (03-2008). “LC-MS/MS Analysis of Phosphorothioate Oligonucleotides” Pittcon 2008, New Orleans, LA.
  • C.M. Castleberry, L. Sallans, R. Kepler, P.A. Limbach (03-2008). “LC-MS/MS Analysis of Phosphorothioate Oligonucleotides” Fifth Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Kady L. Krivos, Patrick A. Limbach (03-2008). “Electron Capture Dissociation for RNA: Protein Cross-links” Fifth Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Kady L. Krivos, Larry Sallans, Patrick A. Limbach (06-2008). “The Optimal Properties of Cross-linked RNA:Protein for Electron Capture Dissociation” 56th ASMS Conference on Mass Spectrometry and Allied Topics , Denver, CO.
  • C.M. Castleberry, R. Kepler, L. Sallans, R.M. Selby, P.A. Limbach (06-2008). “Developing Approaches for Quantitative LC-MS/MS of Nucleic Acids” 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO.
  • Susan P. Russell and Patrick A. Limbach (06-2008). “Post-transcriptional Modifications in Bacterial rRNA and Heat Shock, A Reproducibility Study” 56th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO.
  • Tiffany Hilson, Patrick A. Limbach (06-2008). "Fractionation of Escherichia coli tRNAs Through Two Dimensional Polyacrylamide Gel Electrophoresis", First Year Graduate Student Poster Session, Cincinnati, OH.
  • R. L. Rohlfs, P. A. Limbach (10-2008). “Ribosome Modifications and Assembly Defects” Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • Kady L. Krivos, Patrick A. Limbach (10-2008). “Electron Transfer Dissociation of RNA: Peptide synthetic cross-links” Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • Tiffany Hilson, Patrick A. Limbach (10-2008). "Fractionation and Extraction of Escherichia coli tRNAs for Quantitative Signature Digestion Product Identification" Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • Balasubrahmanyam Addepalli, Pavanapuresan Vaidyanathan, Arun Malhotra, Patrick A. Limbach (10-2008). “Detection of Pseudouridine Modifications in Helix 69 of Escherichia coli 23S rRNA” Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • R. L. Rohlfs, P. A. Limbach (10-2008). “Ribosome Modifications and Assembly Defects” Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • Susan P. Russell and Patrick A. Limbach (10-2008). “ Heat Shock and Post-Transcriptional Modifications in Bacterial Ribosomal RNA” Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • Kirk Gaston, Kady Krivos, Mary Anne T. Rubio, Patrick A. Limbach and Juan Alfonzo (01-2009). “Editing and modification of Kineotoplastid threonyl-tRNA” RNA Editing Gordon Conference, Galveston, TX.
  • I. Nikcevic, T. Wyrzykiewicz, P. A. Limbach (01-2009). LC-MS method development for the characterization of low level impurities in modified phosphorothioatediesters oligonucleotides synthesized using solid-phase phosphoramidite approach 13th Lab Automation National Meeting 2009, Palms Springs, CA.
  • J. A. Caruso, R. N. Easter, K. E. Lokits. P. A. Limbach (03-2009). “Elemental and molecular mass spectrometries for low-level detection of phosphorothioate antisense drugs” The Pittsburgh Conference, Pittcon, Chicago, IL.
  • B. Addepalli, P. Vaidyanathan, A. Malhotra, P.A. Limbach (03-2009). “Detection of Pseudouridine Modifications in Helix 69 of Escherichia coli 23S rRNA” The Pittsburgh Conference, Pittcon, Chicago, IL.
  • R. N. Easter, P A. Limbach and J.A.Caruso (03-2009). “Separation and identification of polythymidilic acids by hydrophilic interaction liquid chromatography (HILIC) coupled to ICPMS” The Pittsburgh Conference, Pittcon, Chicago, IL.
  • I. Nikcevic, T. Wyrzykiewicz, P. A. Limbach (03-2009). Method development for profiling of impurities in synthetic oligonucleotides synthesized by solid-phase phosphoramidite approach The Pittsburgh Conference, Pittcon, Chicago, IL.
  • I. Nikcevic, T. Wyrzykiewicz, P. A. Limbach (04-2009). Characterization of low level impurities in modified phosphorothioatediesters oligonucleotides synthesized using LC-FTMS 7th North American FTMS Conference, Key West, FL.
  • Kady Krivos and Patrick Limbach (04-2009). ECD vs. ETD Sequencing of RNA: Protein Complexes 7th North American FTMS Conference, Key West, FL.
  • B. Catron, S. Afton, P. Limbach, J. Caruso (04-2009). Advances in Sulfur and Phosphorus Detection via ICPMS: Novel Applications Sixth Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Kady Krivos and Patrick A. Limbach (04-2009). Using Mass Spectrometry to Investigate tRNA Modifications 2009 Industrial Affiliates Meeting at UC, Cincinnati, OH.
  • Rebecca L. Rohlfs, Steven Gregory, Al E. Dahlberg, Patrick. A. Limbach (05-2009). “Ribosome Modifications and Assembly Defects” Fourteenth Annual Meeting of the RNA Society, Madison, WI.
  • S. Russell, P. A. Limbach (05-2009). Measuring Global Changes in Post-transcriptionally Modified Nucleosides Induced by Heat Shock Fourteenth Annual Meeting of the RNA Society, Madison, WI.
  • R. N. Easter, K. K. Kroening, K. E. Lokits, P. A. Limbach and J. A. Caruso (06-2009). Separation and Quantification of antisense oligonucleoides by hydrophilic interaction liquid chromatography coupled to ICP-MS 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA.
  • B. Addepalli, A.G. Hunt, P.A. Limbach (06-2009). Using Mass Spectrometry to Characterize the Novel Disulfide Linkage in the Endonuclease Motif of Arabidopsis CPSF30 Ortholog 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA.
  • Kady Krivos and Patrick A. Limbach (06-2009). "CID vs. ECD/ETD Sequencing of RNA:Protein Complexes" 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA.
  • R.P. Dator, R. L. Rohlfs, P.A. Limbach (06-2009). Using Mass Spectrometry to Identify Components of Ribosome Assembly Intermediates 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA.
  • Siwei Li, C.M. Castleberry, P.A. Limbach (06-2009). Characterizing Quantifiable Signature Digestion Products of tRNAs by LC-MS/MS 57th ASMS Conference on Mass Spectrometry and Allied Topics, Philadelphia, PA.
  • R. N. Easter, K. K. Kroening, K. E. Lokits, P. A. Limbach and J. A. Caruso (06-2009). Separation and Quantification of antisense oligonucleoides by hydrophilic interaction liquid chromatography coupled to ICP-MS 2nd International Symposium on Metallomics, Cincinnati, OH.
  • B. Catron, S. Afton, P. Limbach, J. Caruso (06-2009). Advances in Sulfur and Phosphorus Detection via ICPMS for the identification of oliogonucleotide: peptide heteroconjugates 2nd International Symposium on Metallomics, Cincinnati, OH.
  • A.G. Hunt, S. Rao, R.D. Dinkins, B. Addepalli, P.A. Limbach (08-2009). Novel characteristics of the Arabidopsis polyadenylation factor subunit CPSF30 2009 Cold Spring Harbor Laboratory Meeting on Eukaryotic mRNA Processing, Cold Spring Harbor, NY.
  • Nicki Reishus, Kady Krivos, Patrick A. Limbach (08-2009). Incorporating increased amounts of 4-thiouridine into bacterial rRNA University of Cincinnati 2009 REU Poster Session, Cincinnati, OH.
  • Annika Butterer, Colette M. Castleberry, Patrick A. Limbach (08-2009). Immobilizing ribonucleases for qualitative and quantitative analysis of tRNAs University of Cincinnati 2009 REU Poster Session, Cincinnati, OH.
  • B. Addepalli, A.G. Hunt, P.A. Limbach (10-2009). Disulfide Linkage in the Endonuclease Motif of Arabidopsis CPSF30 Ortholog Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • Susan Russell, Kady Krivos, Colette Castleberry, Patrick Limbach (10-2009). Agmatidine is the Modified Cytidine in the Anticodon of tRNAIle in Archaea that Base-Pairs with Adenosine but not with Guanosine Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • Rebecca L. Rohlfs, Romel Dator, Steven Gregory, Al E. Dahlberg, Patrick. A. Limbach (10-2009). “Ribosome Modifications and Assembly Defects” Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • T. D. Hilson, P.A. Limbach (10-2009). Developing and Optimizing and a Mass Spec-Friendly Protocol for Fractionation of tRNAs Through Two-Dimensional Polyacrylamide Gel Electrophoresis Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • R.P. Dator, R. L. Rohlfs, P.A. Limbach (10-2009). Isolation and Characterization of Ribosome Assembly Intermediates Rustbelt RNA Meeting, Deer Creek State Park and Resort, Mt Sterling OH.
  • Susan Russell, Kady Krivos, Colette Castleberry, Patrick Limbach (10-2009). Agmatidine is the Modified Cytidine in the Anticodon of tRNAIle in Archaea that Base-Pairs with Adenosine but not with Guanosine Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • Rebecca L. Rohlfs, Romel Dator, Steven Gregory, Al E. Dahlberg, Patrick. A. Limbach (10-2009). “Ribosome Modifications and Assembly Defects” Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • R.P. Dator, R. L. Rohlfs, P.A. Limbach (10-2009). Isolation and Characterization of Ribosome Assembly Intermediates Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • T. D. Hilson, P.A. Limbach (10-2009). Developing and Optimizing and a Mass Spec-Friendly Protocol for Fractionation of tRNAs Through Two-Dimensional Polyacrylamide Gel Electrophoresis Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • Catron, Brittany; Afton, Scott; Limbach, Patrick; Caruso, Joseph A. (01-2010). Utilizing capillary HPLC-ICPMS for identification of oligonucleotide:peptide heteroconjugates Winter Conference on Plasma Spectrochemistry, Fort Meyers, Florida.
  • Balasubrahmanyam Addepalli, Patrick Limbach (01-2010). Mass Spectrometry based detection of pseudouridine in RNA 22nd Sanibel Conference on Mass Spectrometry: From Structural Biology to Drug Discovery: New Roles for Mass Spectrometry of Nucleic Acids, St. Pete Beach, FL.
  • Rebecca L. Rohlfs, Romel Dator, Patrick. A. Limbach (01-2010). “Ribosome Modifications and Assembly Defects” 22nd Sanibel Conference on Mass Spectrometry: From Structural Biology to Drug Discovery: New Roles for Mass Spectrometry of Nucleic Acids, St. Pete Beach, FL.
  • Susan P Russell, Patrick A. Limbach, Brian P. Anton, Elisabeth A. Raleigh and Richard Roberts (01-2010). Characterization of the YmcB and YqeV tRNA Methylthiotransferases of Bacillus subtilis 22nd Sanibel Conference on Mass Spectrometry: From Structural Biology to Drug Discovery: New Roles for Mass Spectrometry of Nucleic Acids, St. Pete Beach, FL.
  • Catron, Brittany; Caruso, Joseph; Giliberti, Jacqueline; Janssen, Gary; Limbach, Patrick A. (04-2010). Detection of Peptide-Oligonucleotide Heteroconjugates and Protein:RNA Cross-links via Capillary ICPMS Seventh Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Romel Dator, Patrick Limbach (04-2010). Mass Spectrometry-based Characterization of Antibiotic-Induced Ribosome Assembly Intermediates Seventh Annual Ohio Valley Mass Spectrometry Symposium, Columbus, OH.
  • Catron, Brittany; Caruso, Joseph; Giliberti, Jacqueline; Janssen, Gary; Limbach, Patrick A. (05-2010). Detection of Peptide-Oligonucleotide Heteroconjugates and Protein:RNA Cross-links via Capillary ICPMS 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, UT.
  • Siwei Li, Colette Castleberry, Patrick A. Limbach (05-2010). Monitoring Changes in the Expression Levels of Transfer RNAs by LC-MS 58th ASMS Conference on Mass Spectrometry and Allied Topics, Salt Lake City, UT.
  • Balasubrahmanyam Addepalli, Patrick Limbach (06-2010). Quantification of pseudouridine in RNA 15th Annual Meeting of the RNA Society, Seattle, WA.
  • Romel Dator, Patrick Limbach (06-2010). Mass Spectrometry-based Characterization of Ribosome Assembly Intermediates 15th Annual Meeting of the RNA Society, Seattle, WA.
  • Rebecca L. Rohlfs, Romel Dator, Rachel Helpling, Patrick A. Limbach (06-2010). “Ribosome Modifications and Assembly Defects” 15th Annual Meeting of the RNA Society, Seattle, WA.
  • Susan P. Russell and Patrick A. Limbach (06-2010). Measuring Global Changes in Post-transcriptionally Modified Nucleosides Induced by Heat Shock 15th Annual Meeting of the RNA Society, Seattle, WA.
  • M. Zorc, K. Krivos, C. Wetzel, P.A. Limbach (09-2010). Elimination of Ribonuclease Contamination in Transfer RNA Sequencing 2010 University of Cincinnati Women in Science and Engineering Program, Cincinnati, OH.
  • Romel Dator, Patrick Limbach (10-2010). Characterization of Improperly Folded Ribosome Assembly Intermediates from Ribonuclease E Mutants Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • Siwei Li, Colette Castleberry, Patrick A. Limbach (10-2010). Monitoring Changes in the Expression Levels of Transfer RNAs by LC-MS Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • Collin Wetzel, Patrick. A. Limbach (10-2010). HPLC vs. UPLC tandem mass spectrometry for the detection of transfer ribonucleic acid (tRNA) signature digestion products Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.
  • Romel Dator, Patrick Limbach (10-2010). Characterization of Improperly Folded Ribosome Assembly Intermediates from Ribonuclease E Mutants Rustbelt RNA Meeting, Cleveland, OH.
  • Siwei Li, Patrick A. Limbach (10-2010). Comparative Identification of Sequence Variation in tRNAs from Two Organisms by LC-MS” Rustbelt RNA Meeting, Cleveland, OH.
  • Collin Wetzel, Patrick. A. Limbach (10-2010). HPLC vs. UPLC tandem mass spectrometry for the detection of transfer ribonucleic acid (tRNA) signature digestion products Rustbelt RNA Meeting, Cleveland, OH.
  • Balasubrahmanyam Addepalli, Patrick Limbach, (01-2011). Quantification of Pseudouridine in RNA RNA Editing: Editing and Modification of RNA and DNA (Gordon-Kenan Research Seminar), Galveston Island Texas.
  • Brittany Catron, Joseph Caruso, Jacqueline Giliberti, Gary Janssen, Patrick Limbach (01-2011). Detection of Peptide-Oligonucleotide Heteroconjugates and Protein:RNA Cross-links via Capillary ICPMS 2011 Winter Conference on Plasma Spectrochemistry, Zarazoga, Spain.
  • Romel Dator, Patrick Limbach (04-2011). Mass Spectrometry-based Characterization of Antibiotic-Induced Ribosome Assembly Intermediates 8th Annual Ohio Mass Spectrometry Symposium, Columbus, OH.
  • Siwei Li, Patrick A. Limbach (04-2011). A New Method for Sequencing Ribonucleic Acids (RNAs) Using Isotope Labeling and LC-MS/MS 8th Annual Ohio Mass Spectrometry Symposium, Columbus, OH.
  • Collin Wetzel, Patrick. A. Limbach (04-2011). HPLC vs. UPLC tandem mass spectrometry for the detection of transferribonucleic acid (tRNA) signature digestion products 8th Annual Ohio Mass Spectrometry Symposium, Columbus, OH.
  • Brittany Catron, Joseph Caruso, Jacqueline Giliberti, Gary Janssen, Patrick Limbach (06-2011). Analysis of Ribosomal Protein S1 and Messenger Ribonucleic Acid (mRNA) Cross-links by ICPMS and LC-MS/MS 59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO.
  • Balasubrahmanyam Addepalli, Patrick Limbach (06-2011). Pseudouridine Quantification in Transfer and Ribosomal RNAs by Selected Reaction Monitoring Mass Spectrometry 59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO.
  • Siwei Li, Patrick A. Limbach (06-2011). A New Method for Sequencing Ribonucleic Acids (RNAs) Using Isotope Labeling and LC-MS/MS 59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO.
  • Rebecca L. Rohlfs, Patrick A. Limbach (06-2011). Site-Specific Quantification of Ribosomal RNA Modification Profiles by Mass Spectrometry 59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO.
  • Romel Dator, Patrick Limbach (06-2011). Using Mass Spectrometry to Identify the Components of the Erythromycin-Induced 50S Ribosomal Subunit Assembly Particle in Escherichia coli 59th ASMS Conference on Mass Spectrometry and Allied Topics, Denver, CO.
  • Lihua Fu, Patrick A. Limbach (10-2011). Identification of tRNA modifications in Thermus thermophilus HB8 wild type and ΔTTHA1897 mutant strains 2011 Rustbelt RNA Meeting, Dayton, OH.
  • Collin Wetzel, Patrick. A. Limbach (10-2011). Global identification of tRNA through the targeted detection of unique transitions 2011 Rustbelt RNA Meeting, Dayton, OH.
  • Kirk W. Gaston, Gabriele Phillips, Valerie De Crecy-Lagard, and Patrick A. Limbach (10-2011). Archaeosine Synthesis Pathways and Production in E. coli tRNAs 2011 Rustbelt RNA Meeting, Dayton, OH.
  • Siwei Li, Patrick A. Limbach (10-2011). A New Method for Sequencing Ribonucleic Acids (RNAs) Using Isotope Labeling and LC-MS/MS 2011 Rustbelt RNA Meeting, Dayton, OH.
  • Romel Dator, Patrick Limbach (10-2011). Characterizing ribosome biogenesis and assembly using mass spectrometry 2011 Rustbelt RNA Meeting, Dayton, OH.
  • Lihua Fu, Patrick A. Limbach (10-2011). Identification of tRNA modifications in Thermus thermophilus HB8 wild type and ΔTTHA1897 mutant strains Ralph and Helen Oesper Symposium/ACS Cincinnati Local Section Meeting, Cincinnati, OH.
  • Brittany Catron, Joseph Caruso, Jacqueline Giliberti, Gary Janssen, Patrick Limbach (10-2011). Analysis of Ribosomal Protein S1 and Messenger Ribonucleic Acid (mRNA) Cross-links by ICPMS and LC-MS/MS Ralph and Helen Oesper Symposium/ACS Cincinnati Local Section Meeting, Cincinnati, OH.
  • Collin Wetzel, Patrick. A. Limbach (10-2011). Global identification of tRNA through the targeted detection of unique transitions Ralph and Helen Oesper Symposium/ACS Cincinnati Local Section Meeting, Cincinnati, OH.
  • Romel Dator, Patrick Limbach (10-2011). Characterizing ribosome biogenesis and assembly using mass spectrometry Ralph and Helen Oesper Symposium/ACS Cincinnati Local Section Meeting, Cincinnati, OH.
  • Rebecca L. Rohlfs, Patrick A. Limbach (01-2011). Site-Specific Quantification of Ribosomal RNA Modification Profiles by Mass Spectrometry Gordon-Kenan Research Seminar on Editing and Modification of RNA and DNA, Galveston Island Texas.
  • Romel P. Dator and Patrick A. Limbach (03-2012). Characterizing Ribosome Biogenesis and Assembly Using Mass Spectrometry UC Graduate Poster Forum, University of Cincinnati.
  • Siwei Li and Patrick A. Limbach (05-2012). Identification of tRNA Sequence Isomer by Isotope Labeling and LC-MS/MS 60th ASMS conference on Mass Spectrometry and Allied Topics, Vancouver, Canada.
  • Collin Wetzel and Patrick A. Limbach (05-2012). Targeted Tandem Mass Spectrometry for tRNA Identification 60th ASMS conference on Mass Spectrometry and Allied Topics, Vancouver, Canada.
  • Romel P. Dator and Patrick A. Limbach (05-2012). Understanding Mass Spectrometry-based Quantitation of Proteins in Ribonucleoprotein Complexes 17th Annual Meeting of the RNA Society, Ann Arbor, MI.
  • S. Y. Wong, B. Javid, B. Addepalli, G. Piszczek, M. B. Strader, P. A. Limbach, C. Barry (06-2012). Molecular mechanism of streptomycin resistance in Mycobacterium tuberculosis due to mutations in GidB, a 16S rRNA methyltransferase American Society for Microbiology General Meeting, San Francisco, CA.
  • Romel P. Dator and Patrick A. Limbach (09-2012). Mass Spectrometry-based Quantitation of Proteins in Ribonucleoprotein Complexes 11th European Conference on Computational Biology, Basel, Switzerland.
  • B. Addepalli, K. Eileen, S. Gregory, P.A. Limbach (10-2012). Comparison of transfer RNA nucleosides from Deinococcus radiodurans and D. saxicola Rustbelt RNA Meeting, Dayton, OH.
  • Kirk W. Gaston and Patrick A. Limbach (10-2012). Photo-activated Oxidation of tRNA with Riboflavin Rustbelt RNA Meeting, Dayton, OH.
  • Siwei Li and Patrick A. Limbach (10-2012). Automated Analysis of LC-MS data from Isotopically Labeled RNA Rustbelt RNA Meeting, Dayton, OH.
  • Romel P. Dator and Patrick A. Limbach (10-2012). Mass Spectrometry-based Quantitation of Proteins in Ribonucleoprotein Complexes Rustbelt RNA Meeting, Dayton, OH.
  • Romel P. Dator and Patrick A. Limbach (10-2012). Mass Spectrometry-based Quantitation of Proteins in Ribonucleoprotein Complexes Ralph and Helen Oesper Symposium, Cincinnati, OH.
  • Carolin A. Aldinger, Anne-Katrin Leisinger, Kirk W. Gaston, Patrick A. Limbach, Gabor L. Igloi (12-2012). The absence of A-to-I editing in the anticodon of plant cytoplasmic tRNAArgACG demands a relaxation of the wobble rules in plants XXIV tRNA Conference, Olmué, Chile.
  • John J. Perona, Annia Rodriguez-Hernandez, Jessica Spears, Kirk W. Gaston, Patrick A. Limbach, Howard Gamper, Ya-Ming Hou, Paul Agris (12-2012). Structural and mechanistic basis for enhanced translational efficiency by 2-thiouridine at the tRNA anticodon wobble position XXIV tRNA Conference, Olmué, Chile.
  • Robert Ross, Kirk W. Gaston, Balu Addepalli and Patrick A. Limbach (10-2012). Thermus thermophilus tRNAs Asn, Asp, His, and Tyr contain guanosine rather than queuosine at position 34 Rustbelt RNA Meeting, Dayton, OH.
  • Robert Ross, Kirk W. Gaston, Balu Addepalli and Patrick A. Limbach (10-2012). Thermus thermophilus tRNAs Asn, Asp, His, and Tyr contain guanosine rather than queuosine at position 34 Ralph and Helen Oesper Symposium, University of Cincinnati.
  • Wunan Shi, Collin Wetzel and Patrick A. Limbach (03-2012). Total tRNA Mapping in Thermus thermophulis Using LC-MS/MS UC Graduate Poster Forum, University of Cincinnati.
  • Wunan Shi, Collin Wetzel and Patrick A. Limbach (10-2012). High throughput De Novo Sequencing of Total tRNAs in Thermus thermophulis Using LC-MS/MS Ralph and Helen Oesper Symposium, University of Cincinnati.
  • Wunan Shi, Collin Wetzel and Patrick A. Limbach (10-2012). High throughput De Novo Sequencing of Total tRNAs in Thermus thermophulis Using LC-MS/MS Rustbelt RNA Meeting, Dayton, OH.
  • Siwei Li and Patrick A. Limbach (10-2012). Automated Analysis of LC-MS data from Isotopically Labeled RNA Ralph and Helen Oesper Symposium, University of Cincinnati.
  • Kirk W. Gaston and Patrick A. Limbach (01-2013). Photo-activated Oxidation of tRNA with Riboflavin Gordon Research Conference: RNA Editing and Modification, Galveston Island, TX.
  • Siwei Li and Patrick A. Limbach (04-2013). Automated Comparative Analysis of LC-MS datasets from Isotopically Labeled RNA 2013 International Conference of RNA Nanotechnology and Therapeutics, Lexington, KY.
  • Collin Wetzel and Patrick. A. Limbach (04-2013). Targeted Tandem Mass Spectrometry for tRNA Identification 2013 International Conference of RNA Nanotechnology and Therapeutics, Lexington, KY.
  • S. Kesanam, B. Addepalli, P. A. Limbach (04-2013). Overexpression and Purification of the RNA-specific hydrolytic enzyme RNase U2 University of Cincinnati Undergraduate Research Conference, Cincinnati OH.
  • B. Addepalli and P. A. Limbach (06-2013). Method for pseudouridine detection and quantification in RNA by chemical derivatization and tandem mass spectrometry 61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN.
  • Romel P. Dator, Kirk W. Gaston, Patrick A. Limbach (06-2013). Quantitation of Bacterial Ribosomal Proteins by Mass Spectrometry 61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN.
  • Kirk W. Gaston and Patrick A. Limbach (06-2013). Mass spectral analysis of modified nucleosides and nucleoside monophosphates 61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN.
  • Siwei Li and Patrick A. Limbach (06-2013). High Throughput Analysis of LC-MS datasets from Isotopically Labeled RNA 61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN.
  • Robert Ross and Patrick A. Limbach (06-2013). Thermus thermophilus tRNAs Asn, Asp, His, and Tyr contain guanosine rather than queuosine at position 34 61st ASMS conference on Mass Spectrometry and Allied Topics, Minneapolis MN.
  • Romel P. Dator, Kirk W. Gaston, Patrick A. Limbach (07-2013). Characterizing Ribosomes and Ribosome Assembly Complexes by Mass Spectrometry Ribosomes Conference, Napa Valley, CA,.
  • T. Chen, B. Addepalli, N. Shkriabai, M. Kvaratskhelia, P. A. Limbach, V. Gopalan (10-2013). Mass spectrometry-aided identification of the contact sites between a precursor tRNA and a proteinaceous RNase P from Arabidopsis thaliana Finger Lakes RNA Conference, Canandaigua NY.
  • Xiaoyu Cao, Patrick A. Limbach (10-2013). The Posttranscriptionally Modified Nucleosides of Streptomyces griseus tRNAs Ralph and Helen Oesper Symposium, Cincinnati, OH.
  • Y. Jiao, P. A. Limbach (10-2013). Ligating Nicotinamide Mononucleotide to Transfer RNA Digestion Products by T4 RNA Ligase Ralph and Helen Oesper Symposium, Cincinnati, OH.
  • Whitney Houser and Patrick A. Limbach (10-2013). LC-MS/MS analysis of tRNA using digestion with RNase U2 and Lambda Protein Phosphatase Ralph and Helen Oesper Symposium, Cincinnati, OH.
  • Katherine M. Anderson, Ian M.C. Fleming, Kirk W. Gaston, Pat A. Limbach, Mary Anne Rubio, Juan D. Alfonzo (10-2013). Functional dependence of a tRNA editing deaminase and a methyltransferase in Trypanosoma brucei Rustbelt RNA Meeting, Cleveland, OH.
  • Xiaoyu Cao, Patrick A. Limbach (10-2013). The Posttranscriptionally Modified Nucleosides of Streptomyces griseus tRNAs Rustbelt RNA Meeting, Cleveland, OH.
  • T. Chen, B. Addepalli, N. Shkriabai, M. Kvaratskhelia, P. A. Limbach, V. Gopalan (10-2013). Mass spectrometry-aided identification of the contact sites between a precursor tRNA and a proteinaceous RNase P from Arabidopsis thaliana Rustbelt RNA Meeting, Cleveland, OH.
  • Kirk W. Gaston, Caroline Köhrer, Debabrata Mandal, Henri Grosjean, Patrick A. Limbach, Uttam L. RajBhandary (10-2013). Life without tRNA Ile-lysidine synthetase: Translation of the isoleucine codon AUA without the canonical tRNA Ile Rustbelt RNA Meeting, Cleveland, OH.
  • Paul J. Sample, Ludek Koreny, Zdenek Paris, Kirk W. Gaston, Pat Limbach, Juan D. Alfonzo (10-2013). A common modification in an unusual place: Wybutosine within the Trypanosome mitochondrion Rustbelt RNA Meeting, Cleveland, OH.
  • Collin Wetzel, Patrick. A. Limbach (10-2013). Improving Comparative Analysis of tRNA by Mass Spectrometry Using Isotopic enrichments Rustbelt RNA Meeting, Cleveland, OH.

Honors & Awards

  • American Society for Mass Spectrometry Research Award Sponsored by Finnigan, LSU, 1998.
  • Peter A. Soderbergh Award for Innovation in Teaching, LSU, 1998.
  • Award of Excellence in Undergraduate Teaching, College of Basic Sciences, LSU, 1999.
  • Nontenured Faculty Award in the Physical Sciences, Phi Kappa Phi, LSU , 1999.
  • Ohio Eminent Scholar in Mass Spectrometry, UC, 2003.
  • Fellow of the Graduate School, UC, 2006.
  • Sigma Xi Young Investigator Research Award, UC, 2008.
  • Cincinnati 2008 Chemist of the Year Award, Cincinnati Section American Chemical Society, UC, 2009.
  • Fellow of the American Association for the Advancement of Science (AAAS), 2013.

Experience & Service

Work Experience

  • 1995 to 1999, Assistant Professor Chemistry, Louisiana State University, Baton Rouge, LA.
  • 1999 to 2001, Associate Professor Chemistry, Louisiana State University, Baton Rouge, LA.
  • 2001 to 2003, Associate Professor Chemistry, University of Cincinnati, Cincinnati, OH.
  • 2003 to Present, Professor Chemistry, University of Cincinnati, Cincinnati, OH.
  • 2005 to 2010, Department Head, Chemistry,.
  • 2013Interim Associate Dean Research and Graduate Affairs, McMicken College of Arts & Sciences.

Post Graduate training and Education

  • 1993 to 1994, Postdoctoral Fellow with Prof. James McCloskey, University of Utah.

Events Organized

  • Conference. ASMS Sanibel Conference (01-2010 to 01-2010) , St. Pete Beach, FL.

Service

  • Editorial Board Member, Journal of the American Society for Mass Spectrometry 01-2005 to 12-2010
  • Editorial Board Member, Analytica Chimica Acta 01-2005 to 12-2015
  • Review Board/Panel, National Institutes of Health NIEHS P30 Center Grant Panel, 08-2010 to 08-2010
  • President, Sigma Xi 07-2012 to 06-2013
  • Center for Clinical & Translational Science & Training (CCTST) Internal Advisory Board, 09-2010
  • Ohio State Proteomics Core Facility Advisory Board External Member, 09-2007
  • Committee Member, UC Faculty Senate Committee on Research and Scholarship, 08-2012 to 07-2014
  • Review Board/Panel, National Institutes of Health ZRR1 BT-7 Special Emphasis Panel, 02/21/2012 to 02/22/2012
  • Review Board/Panel, National Institutes of Health ZRG1-IMST-Q P41 Review Panel, 06/18/2012 to 06/19/2012
  • Review Board/Panel, National Institutes of Health ZRG1 BCMB-D Review Panel, 09/06/2012 to 09/07/2012
  • Review Board/Panel, National Institutes of Health Director’s Pioneer Award 01-2013
  • Review Board/Panel, National Institutes of Health, ZRG1 BBCB-A Panel 04-2013
  • Review Board/Panel, National Science Foundation CAREER Grant Evaluation Panel, 09-2013
  • Sigma Xi Immediate Past President, 07/01/2013 to 06/30/2014

Courses Taught

  • Topics in Biochemistry
    Topics in Biochemistry
    Level: Graduate
    Term: 11A
  • Topics in Biochemistry
    Topics in Biochemistry
    Level: Graduate
    Term: 12W
  • Topics in Biochemistry
    Topics in Biochemistry
    Level: Graduate
    Term: 12S
  • Fundamentals of Mass Spectrometry
    Fundamentals of Mass Spectrometry
    Level: Graduate
    Term: 12S
  • Intermediate Biochemistry: Synthesizing the Molecules of Life
    Intermediate Biochemistry: Synthesizing the Molecules of Life
    Level: Undergraduate
    Term: 12FS
  • Thermodynamics and Molecular Interactions
    Thermodynamics and Molecular Interactions
    Level: Graduate
    Term: 13FS
  • Intermediate Biochemistry: Synthesizing the Molecules of Life
    Intermediate Biochemistry: Synthesizing the Molecules of Life
    Level: Undergraduate
    Term: 13FS
  • Biochemistry Laboratory: Independent Study
    Biochemistry Laboratory: Independent Study
    Level: Undergraduate
    Term: 13SS
  • Modern Topics in Biochemistry
    Modern Topics in Biochemistry
    Level: Graduate
    Term: 13SS