Faculty

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Eric Villegas

Title: Asst Professor - Visiting Adj
Office: 614 Rieveschl Hall
Tel: 513-569-7017
Email: eric.villegas@uc.edu

Education

  • PHD, University of Pennsylvania School of Medicine, Philadelphia, PA, 2001 (Immunology and Parasitology).
  • BA, California State University, Northridge, Northridge, CA, 1994 (Molecular and Cell Biology).

Publications

Peer-reviewed Publications

  • Bushon, R. N., Francy, D. S., Gallardo, V. J., Lindquist, H. D. A., Villegas, E. N., & Ware, M. W. (2013). Reply to "ranking filter methods for concentrating pathogens in lake water".. Applied and Environmental Microbiology, 79 (17), 5420-1.
  • Staggs, S. E., Beckman, E. M., Keely, S. P., Mackwan, R., Ware, M. W., Moyer, A. P., Ferretti, J. A., Sayed, A., Xiao, L., & Villegas, E. N. (2013). The Applicability of TaqMan-Based Quantitative Real-Time PCR Assays for Detecting and Enumerating Cryptosporidium spp. Oocysts in the Environment.. PloS One, 8 (6), e66562.
  • Francy, D. S., Stelzer, E. A., Brady, A. M. G., Huitger, C., Bushon, R. N., Ip, H. S., Ware, M. W., Villegas, E. N., Gallardo, V., & Lindquist, H. D. A. (2013). Comparison of filters for concentrating microbial indicators and pathogens in lake water samples.. Applied and Environmental Microbiology, 79 (4), 1342-52.
  • Raynal, M., Villegas, E. N., & Nelson, K. L. (2012). Enumeration of viable and non-viable larvated Ascaris eggs with quantitative PCR.. Journal of Water and Health, 10 (4), 594-604.
  • Weilhammer, D. R., Iavarone, A. T., Villegas, E. N., Brooks, G. A., Sinai, A. P., & Sha, W. C. (2012). Host metabolism regulates growth and differentiation of Toxoplasma gondii.. International Journal For Parasitology, 42 (10), 947-59.
  • Rhodes, E. R., Villegas, L. F., Shaw, N. J., Miller, C., & Villegas, E. N. (2012). A modified EPA Method 1623 that uses tangential flow hollow-fiber ultrafiltration and heat dissociation steps to detect waterborne Cryptosporidium and Giardia spp.. Journal of Visualized Experiments : JoVE (65).
  • See, M. J., Staggs, S. E., Dubey, J. P., & Villegas, E. N. (2012). Evaluation of four RNA extraction methods for gene expression analyses of Cryptosporidium parvum and Toxoplasma gondii oocysts.. Journal of Microbiological Methods, 89 (3), 185-92.
  • Ryu, H., Tran, H., Ware, M. W., Iker, B., Griffin, S., Egorov, A., Edge, T. A., Newmann, N., Villegas, E. N., & Domingo, J. W. S. (2011). Application of leftover sample material from waterborne protozoa monitoring for the molecular detection of Bacteroidales and fecal source tracking markers.. Journal of Microbiological Methods, 86 (3), 337-43.
  • Ware, M. W., Augustine, S. A. J., Erisman, D. O., See, M. J., Wymer, L., Hayes, S. L., Dubey, J. P., & Villegas, E. N. (2010). Determining UV inactivation of Toxoplasma gondii oocysts by using cell culture and a mouse bioassay.. Applied and Environmental Microbiology, 76 (15), 5140-7.
  • Villegas, E. N., Augustine, S. A. J., Villegas, L. F., Ware, M. W., See, M. J., Lindquist, H. D. A., Schaefer, F. W., & Dubey, J. P. (2010). Using quantitative reverse transcriptase PCR and cell culture plaque assays to determine resistance of Toxoplasma gondii oocysts to chemical sanitizers.. Journal of Microbiological Methods, 81 (3), 219-25.
  • Massie, G. N., Ware, M. W., Villegas, E. N., & Black, M. W. (2010). Uptake and transmission of Toxoplasma gondii oocysts by migratory, filter-feeding fish.. Veterinary Parasitology, 169 (3-4), 296-303.
  • Ware, M. W., & Villegas, E. N. (2010). Improved Cryptosporidium parvum oocyst propagation using dexamethasone suppressed CF-1 mice.. Veterinary Parasitology, 168 (3-4), 329-31.
  • Staggs, S. E., See, M. J., Dubey, J. P., & Villegas, E. N. (2009). Obtaining highly purified Toxoplasma gondii oocysts by a discontinuous cesium chloride gradient.. Journal of Visualized Experiments : JoVE (33).
  • Brescia, C. C., Griffin, S. M., Ware, M. W., Varughese, E. A., Egorov, A. I., & Villegas, E. N. (2009). Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts.. Applied and Environmental Microbiology, 75 (21), 6856-63.
  • Yang, W., Lindquist, H. D. A., Cama, V., Schaefer, F. W., Villegas, E., Fayer, R., Lewis, E. J., Feng, Y., & Xiao, L. (2009). Detection of Toxoplasma gondii oocysts in water sample concentrates by real-time PCR.. Applied and Environmental Microbiology, 75 (11), 3477-83.
  • Matthews, S., Ginzl, D., Walsh, D., Sherin, K., Middaugh, J., Hammond, R., Bodager, D., Komatsu, K., Weiss, J., Pascoe, N., Marciano-Cabral, F., Villegas, E., Visvesvara, G., Yoder, J., Eddy, B., Capewell, L., Sriram, R., Bandyopadhyay, K., Qvarnstrom, Y., DaSilva, A., Johnston, S., Xiao, L., Hill, V., Roy, S., & Beach, M. J. (2008). Primary amebic meningoencephalitis - Arizona, Florida, and Texas, 2007 . MMWR, 57 (21 ), 573-577.
  • Yang, W., Chen, P., Villegas, E. N., Landy, R. B., Kanetsky, C., Cama, V., Dearen, T., Schultz, C. L., Orndorff, K. G., Prelewicz, G. J., Brown, M. H., Young, K. R., & Xiao, L. (2008). Cryptosporidium source tracking in the Potomac River watershed. Appl Environ Microbiol, 74 (21 ), 6495-504.
  • Villegas, E. N., Glassmeyer, S. T., Ware, M. W., Hayes, S. L., & Schaefer, F. W. (2007). Matrix-assisted laser desorption/ionization time of flight mass spectrometry-based analysis of Giardia lamblia. J Eukaryot Microbiol, 54 (2 ), 55S-55S.
  • Villegas, E. N., Glassmeyer, S. T., Ware, M. W., Hayes, S. L., & Schaefer, F. W. (2006). Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry-Based Analysis of Giardia lamblia and Giardia muris. J Eukaryot Microbiol, 53 Suppl 1 (11 ), S179-81.
  • Lieberman, L. A., Villegas, E. N., & Hunter, C. A. (2004). Interleukin-15-deficient mice develop protective immunity to Toxoplasma gondii. Infect Immun, 72 (11 ), 6729-32.
  • Villegas, E. N., Lieberman, L. A., Mason, N., Blass, S. L., Zediak, V. P., Peach, R., Horan, T., Yoshinaga, S., & Hunter, C. A. (2002). A role for inducible costimulator protein in the CD28- independent mechanism of resistance to Toxoplasma gondii.. Journal of Immunology (Baltimore, Md. : 1950), 169 (2), 937-43.
  • Villegas, E. N., Lieberman, L. A., Carding, S. R., & Hunter, C. A. (2002). Susceptibility of interleukin-2-deficient mice to Toxoplasma gondii is associated with a defect in the production of gamma interferon. Infect Immun, 70 (9 ), 4757-61.
  • Villegas, E. N., Lieberman, L. A., Mason, N., Blass, S. L., Zediak, V. P., Peach, R., Horan, T., Yoshinaga, S., & Hunter, C. A. (2002). A role for inducible costimulator protein in the CD28- independent mechanism of resistance to Toxoplasma gondii. J Immunol, 169 (2 ), 937-43.
  • Wille, U., Villegas, E. N., Craig, L., Peach, R., & Hunter, C. A. (2002). Contribution of interleukin-12 (IL-12) and the CD28/B7 and CD40/CD40 ligand pathways to the development of a pathological T-cell response in IL-10-deficient mice. Infect Immun, 70 (12 ), 6940-7.
  • Murphy, M. L., Wille, U., Villegas, E. N., Hunter, C. A., & Farrell, J. P. (2001). IL-10 mediates susceptibility to Leishmania donovani infection. Eur J Immunol, 31 (10 ), 2848-56.
  • Wille, U., Villegas, E. N., Striepen, B., Roos, D. S., & Hunter, C. A. (2001). Interleukin-10 does not contribute to the pathogenesis of a virulent strain of Toxoplasma gondii. Parasite Immunol, 23 (6 ), 291-6.
  • Caamano, J., Tato, C., Cai, G., Villegas, E. N., Speirs, K., Craig, L., Alexander, J., & Hunter, C. A. (2000). Identification of a role for NF-kappa B2 in the regulation of apoptosis and in maintenance of T cell-mediated immunity to Toxoplasma gondii. J Immunol, 165 (10 ), 5720-8.
  • Cai, G., Radzanowski, T., Villegas, E. N., Kastelein, R., & Hunter, C. A. (2000). Identification of STAT4-dependent and independent mechanisms of resistance to Toxoplasma gondii. J Immunol, 165 (5 ), 2619-27.
  • Reichmann, G., Walker, W., Villegas, E. N., Craig, L., Cai, G., Alexander, J., & Hunter, C. A. (2000). The CD40/CD40 ligand interaction is required for resistance to toxoplasmic encephalitis. Infect Immun, 68 (3 ), 1312-8.
  • Villegas, E. N., Wells, A. D., Turka, L. A., & Hunter, C. A. (2000). The role of CD28 in the generation and maintenance of protective T cell responses during toxoplasmosis. Faseb Journal, 14 (6 ), A980-A980.
  • Villegas, E. N., Wille, U., Craig, L., Linsley, P. S., Rennick, D. M., Peach, R., & Hunter, C. A. (2000). Blockade of costimulation prevents infection-induced immunopathology in interleukin-10-deficient mice. Infect Immun, 68 (5 ), 2837-44.
  • Wille, U., Villegas, E. N., Craig, L., Peach, R., & Hunter, C. A. (2000). Blockade of costimulation and IL-12 prevents immunopathology in IL-12 KO mice after infection with T-gondii. Faseb Journal, 14 (6 ), A980-A980.
  • Reichmann, G., Villegas, E. N., Craig, L., Peach, R., & Hunter, C. A. (1999). The CD28/B7 interaction is not required for resistance to Toxoplasma gondii in the brain but contributes to the development of immunopathology. J Immunol, 163 (6 ), 3354-62.
  • Villegas, E. N., Elloso, M. M., Reichmann, G., Peach, R., & Hunter, C. A. (1999). Role of CD28 in the generation of effector and memory responses required for resistance to Toxoplasma gondii. J Immunol, 163 (6 ), 3344-53.
  • Villegas, E. N., Elloso, M. M., Reichmann, G., Peach, R., & Hunter, C. A. (1999). The role of CD28 in resistance to T-gondii infection. Faseb Journal, 13 (5 ), A953-A953.
  • Wille, U., Villegas, E. N., Striepen, B., Roos, D. S., & Hunter, C. A. (1999). The role of IL-10 in the immune response to ELH, a highly virulent strain of Toxoplasma gondii. Faseb Journal, 13 (5 ), A655-A655.
  • Park, C. S., Pardhasaradhi, K., Gianotti, C., Villegas, E., & Krishna, G. (1994). Human retina expresses both constitutive and inducible isoforms of nitric oxide synthase mRNA. Biochem Biophys Res Commun, 205 (1 ), 85-91.
  • Villegas, E., Zareparsi, S., Mora, P., & Jones, K. C. (1992). RESTRICTION ANALYSIS OF THE RDNA REPEAT UNIT OF CHINOOK-SALMON (ONCORHYNCHUS-TSHAWYTSCHA). Molecular Biology of the Cell, 3, A80-A80.

Experience & Service

Post Graduate training and Education

  • 2002 to 2005, Post-doctoral Research Fellow, Cancer Research Laboratories, Immunology Division, University of California, Berkley, Berkeley, CA.
  • 1997 to 1997, Student, Marine Biological Laboratories, WoodsHole, MA.