Faculty

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Brian Kinkle

Title: Professor
Office: 603B Rieveschl Hall
Tel: 513-556-9756
Email: brian.kinkle@uc.edu

Education

  • PhD, University of Minnesota, to Present.

Research Information

Research Interests


My general research interests lie in the overlapping areas of microbial ecology and environmental microbiology. Specific areas of active research include the community analysis and ecological genetics of xenobiotic-degrading and metal-transforming microorganisms in soil (in collaboration with Jodi Shann, Department of Biological Sciences), chemoautotrophic bacteria in groundwater communities (in collaboration with Tom Kane, Department of Biological Sciences), and azo dye-degrading bacteria in bioreactors (in collaboration with Paul Bishop, Department of Environmental Engineering).

Recent advances in molecular biology have provided us with powerful tools that can be used to examine the ecology of microorganisms in natural environments such as soil or water. My laboratory is currently employing techniques such as epifluorescence microscopy, using fluorescent antibodies and ribosomal RNA probes for the detection and enumeration of specific bacteria in natural systems, and in situ DNA hybridization using degradative and heavy metal-resistance gene probes for detection and enumeration of specific bacterial genotypes. We also use ribosomal RNA gene sequencing for applications in microbial phylogeny and ecology.

Research Support

  • (Collaborator), Kinkle, Brian; Oerther, Daniel, Bacterial Diversity as a BioMarker of Soil Health, National Institute of Environmental Health Sciences. (1 R01 ES015448-01), $934,398.00. 08/01/2006 to 05/31/2010. Status: Active.
  • (PI), Kinkle, Brian, Biofilm and Regrowth Issues for Selected Microbial Contaminants, Environmental Protection Agency. (PO 197014), $75,497.00. 06/01/2000 to 05/31/2003. Status: Closed.

Publications

Peer Reviewed Publications

  • Coughlin, M.F., Kinkle, B.K., & Bishop, P.L. (2003). High performance degradation of azo dye Acid Orange 7 and sulfanilic acid in a laboratory scale reactor after seeding with cultured bacterial strains. Water Research, 37, 2757-2763.
  • Rodgers, M., Flanigan, D., Pfaller, S., & Kinkle, B.K. (2003). Identification of a Flavobacterium strain virulent against Giardia lamblia cysts. World J. Microbio. Biotech., 19, 703-709.
  • Popa R., Badescu, A., & Kinkle, B.K. (2004). Pyrite framboids as biomarkers for iron sulfur systems. Geomicrobiol. J., 21, 193-206.
  • Popa, R., & Kinkle, B.K. (2004). Isolation of Thiomonas thermosulfatus strain 51, a species capable of coupling biogenic pyritization with chemiosmotic energy transduction. Geomicrobiol. J., 21, 297-309.
  • Jing, G., Hollis, G., Polaczyk, A., Eluru, H., Kinkle, B., Mast, D., Oerther, D., & Papautsky, I. (2004). Developing rapid detection of mycobacteria using microwaves. Analyst, 129, 963-969.
  • Jing, G., Eluru, H., Polaczyk, A., Kinkle, B., Oerther, D., & Papautsky, I. (2005). Paraffin surfaces for culture-based detection of mycobacteria in environmental samples. J. Micromech. Microeng. (15), 270-276.
  • Revetta, R.P., Rodgers, M.R., & Kinkle, B.K. (2005). Isolation and identification of freshwater bacteria antagonistic to Giardia lamblia cysts. J. Water Health, 3, 83-88.
  • Cheung, P.Y., & Kinkle, B.K. (2005). Effects of nutrients and surfactants on pyrene mineralization and Mycobacterium spp. populations in contaminated soil. Soil Biol. Biochem., 37, 1401-1405.
  • Cheung, P.Y., & Kinkle, B.K. (2005). Changes in Mycobacterium spp. population structure and pyrene mineralization in polycyclic aromatic hydrocarbon amended soils. Soil Biol. Biochem., 37, 1929-1937.
  • Jjemba, P.K., Kinkle, B.K., & Shann, J.R. (2006). In-situ enumeration and probing of pyrene-degrading soil bacteria. FEMS Microbiol. Ecol., 55, 287-298.
  • Polaczyk, A., Kinkle, B., Papautsky, I., & Oerther, D.B. (2006). Culture-Based BioMEMS device to track Gordonia in activated sludge. Environ. Sci. Technol., 40, 2269-2274.
  • Mueller, S.R., Vonderheide, A.P., Shann, J.R., & Kinkle, B.K. (2006). Use of SEC-ICP-MS with a collision cell for determining the interactions of chromium with DNA extracted from metal contaminated soil. Anal. Bioanal. Chem., 386, 142-151.
  • Mueller, K.E., Mueller-Spitz, S.R., Henry, H.F., Vonderheide, A.P., Soman, R.S., Kinkle, B.K., & Shann, J.R. (2006). Fate of penta-brominated diphenyl ethers in soil: abiotic sorption, plant uptake, and interspecific plant competition. Environ. Sci. Technol., 40, 6662-6667.
  • Vonderheide, A.P., Mueller-Spitz, S.R., Meija, J., Mueller, K., Welsh, G.L., Kinkle, B., Shann, J., & Caruso, J.A. (2006). Rapid breakdown of brominated flame retardants by soil microorganisms. J. Anal. Atomic Spectrom., 21, 1232-1239.

In Press

  • Mueller, Kevin E, Mueller-Spitz, Sabrina R, Henry, Heather F, Vonderheide, Anne P, Soman, Rajiv S, Kinkle, Brian K, & Shann, Jodi R (2006). Fate of pentabrominated diphenyl ethers in soil: abiotic sorption, plant uptake, and the impact of interspecific plant interactions. Environmental science & technology, 40(21), 6662-7.
  • Mueller-Spitz, Sabrina R, Vonderheide, Anne P, Shann, Jodi R, Caruso, Joseph A, & Kinkle, Brian K (2006). Use of SEC-ICP-MS with a collision cell for determining the interaction of chromium with DNA extracted from metal-contaminated soils. Analytical and bioanalytical chemistry, 386(1), 142-51.
  • Polaczyk, Amy, Kinkle, Brian, Papautsky, Ian, & Oerther, Daniel B (2006). Culture-based MEMS device to track Gordonia in activated sludge. Environmental science & technology, 40(7), 2269-74.
  • Jjemba, Patrick K, Kinkle, Brian K, & Shann, Jodi R (2006). In-situ enumeration and probing of pyrene-degrading soil bacteria. FEMS microbiology ecology, 55(2), 287-98.
  • Boczek, Laura A, Johnson, Clifford H, Rice, Eugene W, & Kinkle, Brian K (2006). The widespread occurrence of the enterohemolysin gene ehlyA among environmental strains of Escherichia coli. FEMS microbiology letters, 254(2), 281-4.
  • Jing, Gaoshan, Hollis, Grant, Polaczyk, Amy, Eluru, Hima B, Kinkle, Brian, Mast, David, Oerther, Daniel B, & Papautsky, Ian (2004). Developing rapid detection of mycobacteria using microwaves. The Analyst, 129(10), 963-9.
  • Coughlin, Michael F, Kinkle, Brian K, & Bishop, Paul L (2003). High performance degradation of azo dye Acid Orange 7 and sulfanilic acid in a laboratory scale reactor after seeding with cultured bacterial strains. Water research, 37(11), 2757-63.
  • Coughlin, Michael F, Kinkle, Brian K, & Bishop, Paul L (2002). Degradation of acid orange 7 in an aerobic biofilm. Chemosphere, 46(1), 11-9.
  • Jing, G., Polaczyk, A., Kinkle, B., Oerther, D.B., & Papautsky, I. Development of a microfluidic biosensor for detection of environmental mycobacteria.

Experience & Service

Service

  • Committee Chair, Space committee, 01-2010 to 12-2010

Courses Taught

  • 15-BIOL-103 BIOLOGY
    Level: Undergraduate
    Term: 10S

  • 15-BIOL-133 MICROBES & SOCIETY
    Level: Undergraduate
    Term: 10S

  • 15-BIOL-101 BIOLOGY
    Level: Undergraduate
    Term: 10A

  • Evidence based teaching in biology
    Evidence based teaching in biology
    Level: Graduate
    Term: 10A