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Patrick A Limbach

Department Head, Professor
Chemistry - Tenure-Track Faculty
429 Rieveschl Hall
513-556-9200
pat.limbach@uc.edu

Professional Summary

Pat Limbach is a Professor and Ohio Eminent Scholar at the University of Cincinnati. He is a bioanalytical chemist with research interests in mass spectrometry, ribonucleoprotein complexes and protein translation. After earning an undergraduate degree from Centre College in 1988, he studied under the direction of Dr. Alan G. Marshall at The Ohio State University. While there, his graduate research focused on instrumentation improvements to Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. He received his PhD from OSU in 1992. He then took a postdoctoral position at the University of Utah working with Dr. James A. McCloskey. While in Utah, he worked in the area of RNA chemistry and nucleic acid mass spectrometry. In 1995, he joined the faculty at Louisiana State University and was promoted to Associate Professor in 1999. In 2001, he moved to his current position in Cincinnati. He has received numerous teaching and research awards including the Young Investigator Research Award from the American Society for Mass Spectrometry.

Education

PhD, Ohio State University, 1992.

Research Interests

mass spectrometry
microfluidics and microfabricated devices
structural investigation of RNA
characterization of ribonucleoprotein complexes
single-cell proteomics

Research Support

Quantification of Macromolecules, Pfizer Global Research Development Fund. $110,000. Funded 01/01/2007 to 03/31/2008.

(PI) Mass Spectrometry Methods for RNA, National Science Foundation. (CHE 0602413); $436,988. Funded 07/01/2006 to 06/30/2009.

(PI) Acquisition of a Hybrid Fourier Transform Mass Spectrometer for Proteomics, National Institutes of Health. (NIH S10 RR019900); $918,973. Funded 04/25/2005 to 04/24/2006.

(PI) Mass Spectrometry of Ribosomal RNA:Protein Interactions, National Institutes of Health. (R01-GM58843); $1,080,000. Funded 12/01/2004 to 11/30/2008.

(PI) Bioassays and Molecular Characterization by Microchip CE, National Institutes of Health. (R01-GM069547); $1,461,868. Funded 01/01/2004 to 12/31/2007.

(co-PI) Mass Spectrometry Consortium for Materials and Medical Research, Ohio Board of Regents Hayes Fund. $179,900. Funded 07/01/2002 to 06/30/2003.

(PI) Collaborative Research: Single Cell Proteomics, National Science Foundation. (DBI-0137924); $230,174. Funded 04/01/2002 to 03/31/2005.

(Head of Protein Analysis Facilities & Services Core) Environmental Genetics, National Institutes of Health. Funded 03/01/2002 to 02/28/2007.

(PI) Identification of Modified Nucleosides in Ribosomal RNA, National Institutes of Health. (R01-GM58843); $696,881. Funded 02/01/1999 to 01/31/2004.

Peer Reviewed Publications

Hossain, M.; Limbach, P.A. "Mass Spectrometry-Based Detection of Transfer RNAs by Their Signature Endonuclease Digestion Products". RNA (2007), 13, 295-303.

Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "Frontal analysis in microchip capillary electrophoresis: a simple and accurate method for determination of protein-DNA dissociation constant". Electrophoresis (2007), 28, 837-842.

Gong, M.; Wehmeyer, K.R.; Stalcup, A.M.; Limbach, P.A.; Heineman, W.R. "Study of injection bias in a simple hydrodynamic injection in microchip capillary electrophoresis". Electrophoresis (2007), 28, 1564-1571.

Nikcevic, I.; Lee, S.-H.; Piruska, A.; Ahn, C.; Ridgway, T.H.; Limbach, P.A.; Wehymeyer, K.R.; Heineman, W.R.; Seliskar, C.J. "Characterization and performance of injection molded poly(methylmethacrylate) microchips for capillary electrophoresis". J. Chrom. A (2007), 1154, 444-453.

Suh, M.J.; Pourshahian, S.; Limbach, P.A. "Developing Limited Proteolysis and Mass Spectrometry for the Characterization of Ribosome Topography". J. Am. Soc. Mass Spectrom. (2007), 18, 1304-1317.

Duderstadt, R.E.; Tsuie, B.M.; Macha, S.F.; Limbach, P.A. "Investigation of Single-Site Zirconium Azaborolinyl Complexes by Laser Desorption Ionization Time-of-Flight Mass Spectrometry". Anal. Chim. Acta (2007), 596, 124-131.

Mecomber, J.S.; Stalcup, A.M.; Hurd, D; Halsall, H.B.; Heineman, W.R.; Seliskar, C.J.; Wehmeyer, K.R.; Limbach, P.A. "Analytical Performance of Polymer-Based Microfluidic Devices Fabricated by CNC Machining". Anal. Chem. (2006), 78, 936-941.

Carr, J.F.; Hamburg, D.-M.; Gregory, S.T.; Limbach, P.A.; Dahlberg, A.E. "Effect of Streptomycin-resistance Mutations on the Post-translational Modification of Ribosomal Protein S12". J. Bacteriol. (2006), 188, 2020-2023.

Meng, Z.; Limbach, P.A. "Mass Spectrometry of RNA: Linking the Genome to the Proteome". Briefings in Functional Genomics & Proteomics (2006), 5, 87-95.

Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Arias, F.; Heineman, W.R. "On-line Sample Preconcentration using Field-Amplified Stacking Injection in Microchip Capillary Electrophoresis". Anal. Chem. (2006), 78, 3730-3737.

Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "On-line Sample Preconcentration by Sweeping with Dodecyltrimethylammonium Bromide in Capillary Zone Electrophoresis". J. Chrom. A (2006), 1125, 263-269.

Gong, M.; Wehmeyer, K.R.; Limbach, P.A.; Heineman, W.R. "Unlimited-volume Electrokinetic Stacking Injection in Sweeping Capillary Electrophoresis Using a Cationic Surfactant". Anal. Chem. (2006), 78, 6035-6042.

McLachlan, A.; Borchers, M.; Velayutham, P.; Wagner, M.; Limbach, P.A. "Characterizing the Reproducibility of a Protein Profiling Method for the Analysis of Mouse Bronchoalveolar Lavage Fluid". J. Proteome Res. (2006), 5, 3059-3065.

Wetherill, Y.B.; Hess-Wilson, J.K.; Comstock, C.E.S.; Shah, S.A.; Buncher, C.R.; Sallans, L.; Limbach, P.A.; Schwemberger, S.; Babcock, G.F.; Knudsen, K.E. "Bisphenol A facilitates bypass of androgen ablation therapy in prostate cancer". Mol Cancer Therapeutics (2006), 5, 3181-3190.

Tummala, R.; Limbach, P.A. "Effect of Sodium Dodecyl Sulfate Micelles on Peptide Mass Fingerprinting by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry". Rapid Commun. Mass Spectrom. (2005), 18, 2031-2035.

Meng, Z.; Limbach, P.A. "Quantitation of Ribonucleic Acids Using ¹⁸O Labeling and Mass Spectrometry". Anal. Chem. (2005), 77, 1891-1895.

Tu, J.; Halsall, H.B.; Seliskar, C.J.; Limbach, P.A.; Arias, F.; Wehmeyer, K.R.; Heineman, W.R. "Estimation of log Pow Values for Neutral and Basic Compounds by Microchip Microemulsion Electrokinetic Chromatography with Indirect Fluorimetric Detection". J. Pharm. Biomed. Anal. (2005), 38, 1-7.

Mecomber, J.S.; Hurd, D.; Limbach, P.A. "Enhanced machining of micron-scale features in microchip molding masters by CNC milling". Int. J. Machine Tools Manufacture (2005), 45, 1542-1550.

Tummala, R.; Green-Church, K.B.; Limbach, P.A. "Interactions Between Sodium Dodecyl Sulfate Micelles and Peptides During Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) of Proteolytic Digests". J. Am. Soc. Mass Spectrom. (2005), 16, 1438-1446.

Meng, Z.; Limbach, P.A. "Shotgun Sequencing Small Oligonucleotides by Use of Nozzle-Skimmer Dissociation and Electrospray Ionization Mass Spectrometry". Eur. J. Mass Spectrom. (2005), 11, 221-229.

Currie, C.C.; Heineman, W.R.; Halsall, H.B.; Seliskar, C.J.; Limbach, P.A.; Arias, F.; Wehmeyer, K.R. "Estimation of pKa Values Using Microchip CE Coupled with Indirect Fluorescence Detection". J. Chrom. B (2005), 824, 201-205.

Tummala, R.; Limbach, P.A. "Serum Protein Profiling Using Surfactant-Aided Matrix Assisted Laser Desorption/Ionization Mass Spectrometry". Anal. Chim. Acta (2005), 551, 137-141.

Meades, G.D. Jr.; McLachlan, A.; Sallans, L.; Limbach, P.A.; Frankel, L.K.; Bricker, T.M. "Association of the 17 kDa Protein with Photosystem II in Higher Plants". Biochemistry (2005), 44, 15216-15221.

Piruska, A.; Nikcevic, I.; Lee, S.-H.; Ahn, C.; Heineman, W.R.; Limbach, P.A.; Seliskar, C.J. "The Autofluorescence of Plastic Materials and Chips Under Laser Irradiation". Lab Chip (2005), 5, 1348-1354.

Suh, M.-J.; Hamburg, D.-M.; Gregory, S.T.; Dahlberg, A.E.; Limbach, P.A. "Extending Ribosomal Protein Identifications to Unsequenced Bacterial Strains Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry". Proteomics (2005), 5, 4818-4831.

Meng, Z.; Simmons-Willis, T.A.; Limbach, P.A. "The Use of Mass Spectrometry in Genomics". Biomolecular Engineering (2004), 21, 1-13.

Suh, M.-J.; Limbach, P.A. "Investigation of Protein Isolation Methods Suitable for the Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Analysis of Ribonucleoprotein Complexes". Eur. J. Mass Spectrom (2004), 10, 89-99.

Meng, Z.; Limbach, P.A. "RNase Mapping of Intact Nucleic Acids by Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (ESI-FTICRMS) and 18O Labeling". Int. J. Mass Spectrom (2004), 234, 37-44.

Cameron, D.M.; Gregory, S.T.; Thompson, J.; Suh, M.-J.; Limbach, P.A.; Dahlberg, A.E. "Thermus thermophilus L11 Methyltransferase, PrmA, is Dispensable for Growth and Preferentially Modifies Free Ribosomal Protein L11 Prior to Ribosome Assembly". J. Bacteriol (2004), 186, 5819-5825.

Berhane, B.T.; Limbach, P.A. "Functional Microfabricated Sample Targets for Matrix-assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry Analysis of Ribonucleic Acids". Anal. Chem. (2003), 75, 1997-2003.

Dominick, W.D.; Berhane, B.T.; Mecomber, J.S.; Limbach, P.A. "Covalent Immobilization of Proteases and Nucleases to Poly(methylmethacrylate)". Anal. Bioanal. Chem (2003), 376, 349-354.

Berhane, B.T.; Limbach, P.A. "Stable Isotope Labeling for Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry and Post-Source Decay Analysis of Ribonucleic Acids". J. Mass Spectrom (2003), 38, 872-878.

Robins, C.R.; Limbach, P.A. "The Use of Nonpolar Matrices for Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Analysis of High Boiling Crude Oil Fractions". Rapid Commun. Mass Spectrom (2003), 17, 2839-2845.

Hassett, D.J.; Limbach, P.A.; Hennigan, R.F.; Klose, K.E.; Hancock, R.E.W.; Platt, M.D.; Hunt, D.F. "Bacterial Biofilms of Importance to Medicine and Bioterrorism: Proteomic Techniques to Identify Novel Vaccine Components and Drug Targets". Exp. Opin. Biol. Therapy (2003), 3, 1201-1207.

Meng, Z.; Limbach, P.A. "Shotgun Sequencing Small Oligonucleotides by Use of Nozzle-Skimmer Dissociation and Electrospray Ionization Mass Spectrometry". Eur. J. Mass Spectrom., 11, 221-229.

Books

Banoub, J.H.; Limbach, P.A. Mass Spectrometry of Nucleosides and Nucleic Acids; Taylor & Francis, Inc., 2008.

Book Chapters

Jin, L.; Rodicio, L.M.; Limbach, P.A. "Principles of Proteomics". In Angiogenesis, Functional and Medicinal Foods; Losso, J.N.; Shahidi, F.; Bagchi, D., Ed(s); Marcel Dekker, Inc., (2007), pp 291-314.

Mecomber, J.S.; Dominick, W.D.; Jin, L.; Limbach, P.A. "BioMEMS Devices for Proteomics". In BioMEMS: Technologies and Applications; Soper, S.A., Ed(s); Taylor & Francis, Inc., (2007).

Patteson, K.; Limbach, P.A. "Nucleic Acids: DNA and RNA Seqeuence Analysis". In Encyclopedia of Mass Spectrometry Volume 3: Biological Applications Part B: Carbohydrates, Nucleic A; Caprioli, R.; Gross, M., Ed(s); Elsevier, Inc., (2005), pp 1-10.

Patteson, K.; Limbach, P.A. "Nucleic Acids: Base Analysis". In Encyclopedia of Mass Spectrometry Volume 3: Biological Applications Part B: Carbohydrates, Nucleic A; Caprioli, R.; Gross, M., Ed(s); Elsevier, Inc., (2005), pp 14-18.

Macha, S.F.; Limbach, P.A. "Analysis of Polymeric Hydrocarbon Materials by Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry". In Analytical Advances in Hydrocarbon Research; Hsu, S., Ed(s); Kluwer Academic/Plenum Publishers: New York, N.Y., (2003), pp 385-404.

Robins, C.L.; Macha, S.F.; Vandell, V.E.; Limbach, P.A. "LDI- and MALDI-Fourier Transform Ion Cyclotron Resonance (FTICR) Mass Spectrometry Analysis of Hydrocarbon Samples". In Analytical Advances in Hydrocarbon Research; Hsu, S., Ed(s); New York, N.Y.: Kluwer Academic/Plenum Publishers, (2003), pp 405-420.

Invited Presentations

(11-2007). Modified RNAs: From Cancer to Biochemistry. Bethany College, Department of Chemistry.

(03-2007). Dissecting RNAs and RNPs in the Gas Phase. Brown University, Department of Microbiology and Biochemistry.

(01-2007). Mass Spectrometry as a Tool to Study RNA and RNA:Protein Complexes. Rennselaer Polytechnic Institute, Department of Chemistry.

(11-2006). Optimizing Surface Chemistries to Develop Plastic Microfluidic and Microarray Devices for Mass Spectrometry. Eastern Analytical Symposium

(11-2005). RNA for the Masses. University of Cincinnati, Department of Biological Sciences.

(06-2005). Developing Mass Spectrometry Approaches for RNA and RNP Complexes. Ohio State University, Department of Molecular Virology, Immunology and Human Genetics.

(04-2005). Protein Factories on Bathroom Scales. John Carroll University, Department of Chemistry.

(11-2004). Microchip Mass Spectrometry: Plastics Make it Possible. Bradley University, Department of Chemistry.

(06-2004). Optimizing Surface Chemistries to Develop Plastic Microfluidic and Microarray Devices for Mass Spectrometry. 87th Canadian Chemistry Conference and Exhibition

(06-2004). Mass Spectrometry as a Tool for Probing Ribosome Structure. trometry as a Tool for Probing Ribosome Structure”,

(04-2004). Profiling the Source of Proteins. University of Oregon, Institute of Molecular Biology.

(04-2004). Developing Mass Spectrometry Approaches for RNP Complexes. University of California, Riverside, Department of Chemistry.

(11-2003). RNA for the Masses: Using Mass Spectrometry to Characterize RNA Structure. 2003 Rustbelt RNA Meeting

(11-2003). Interfacing Microchips to Mass Spectrometry. NSF Workshop on Miniaturization and Mass Spectrometry

(10-2003). Microchip Mass Spectrometry: Plastics Make it Possible. University of Maryland, Baltimore County, Department of Chemistry.

(10-2003). Profiling the Source of Proteins: Mass Spectrometry of the Ribosome. 35th ACS Central Regional Meeting

(09-2003). Bioassays and Molecular Characterization by Microchip CE and Microchip MS. 226th ACS National Meeting

(02-2003). Integrating Microfabricated Devices with Modern Mass Spectrometry. LabAutomation, Palm Springs, CA.

Poster Presentations

M. Hossain, P.A. Limbach (10-2007). Large Scale Quantitive Analysis of Non-coding RNA (ncRNAs) by their Signature Digestion Products using Stable Isotope Labeling and MALDI-MS. NIH 2nd NGSRF 2007, Bethesda, MD.

C.M Castleberry, L.Sallans, and P.A. Limbach (06-2007). Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS. 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.

D.-M Hamburg, M.-Suh and P.A. Limbach (06-2007). Conformational Changes and Chemical Reactivity of the Ribosome: An Application of Limited Proteolysis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.

M. Hossain, P.A. Limbach (06-2007). Global Quantification of tRNAs by their Signature Digestion Products and MALDI-MS. 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (06-2007). Comparing Collision Induced Dissociation and Electron Capture Dissociation for the Sequencing of Peptide:Oligonucleotide Heteroconjugate. 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.

S. Pourshahian, P.A. Limbach (06-2007). Identification of Oligonucleotide-Peptide cross-links using mass defect. ", 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.

R.L. Rohlfs, P.A. Limbach (06-2007). Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the Presence of Antibiotics. 55th ASMS Conference on Mass Spectrometry and Allied Topics, Indianapolis, IN.

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (05-2007). Comparison of Collision Induced Dissociation and Electron Capture Dissociation for the Sequencing of Peptide:Oligonucleotide Heteroconjugate. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.

R.L. Rohlfs, P.A. Limbach (05-2007). Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotics. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.

S. Russell, P.A. Limbach (05-2007). Posttranscriptional Modifications in Bacterial rRNA and Heat Shock. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY.

C.M. Castleberry, L. Sallans, P.A. Limbach (03-2007). Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS. Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.

M. Hossain, P.A. Limbach (03-2007). Quantification of Large Ribosomal RNAs (rRNAs) in Escherichia coli. Graduate Poster Forum, University of Cincinnati.

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (03-2007). Implementing Electron Capture Dissociation as a Method of the Assessment of Cross Linked Protein-Nucleic Acid Complexes. Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.

R.L. Rohlfs, P.A. Limbach (03-2007). Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotic. Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.

Kady L. Krivos, Larry Sallans, Patrick A. Limbach (03-2007). Comparing Collision Induced Dissociation and Electron Capture dissociation for the Assessment of RNA-Peptide Complexes. Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH.

D.-M Hamburg, D.D. Richardson, P.A. Limbach and J.A. Caruso (02-2007). Investigation of Metals in Ribosomal Proteins. European Winter Plasma Conference, Taormino, Italy.

M. Hossain, C. Cherenfant, P.A. Limbach (02-2007). Detection of Cytosolic and Mitochondrial Transfer RNAs of Saccharomyces by their Signature Digestion Products. Pittcon, Chicago, IL.

S. Pourshahian, P.A. Limbach (02-2007). Application of Mass Defect to Identification of Oligonucleotide-Peptide Cross-links. Pittcon, Chicago, IL.

A.Durairaj, P.A. Limbach (10-2006). Identifying Pseudouridines in RNA by Mass Spectrometry. 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.

C.M. Castleberry L. Sallans, P.A. Limbach (10-2006). Quantitation of Isotopically Labeled RNA Using LC-MS. 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.

D.-M Hamburg, M.-J. Suh and P.A. Limbach (10-2006). Probing the Topography Ribonucleoprotein Complexes Using Limited Proteolysis and Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.

M. Hossain, P.A. Limbach (10-2006). Detection of Mitochondrial Transfer RNAs Import of Saccharomyces Cerevisiae by their Signature Digestion Products. 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.

S. Russell, P.A. Limbach (10-2006). Posttranscriptional Modifications in Bacterial rRNA and Heat Shock. 2006 Rustbelt RNA Meeting, Mt. Sterling, OH.

C.M. Castleberry, L. Sallans, P.A. Limbach (10-2006). Quantitation of Isotopically Labeled RNA Using LC-MS. Oesper Symposium, University of Cincinnati, Cincinnati, OH.

Kady L. Krivos, Larry Sallans, Patric A. Limbach (10-2006). Implementing Electron Capture Dissociation as a Method of the Assessment of Cross Linked Protein-Nucleic Acid Complexes. Oesper Symposium, University of Cincinnati, Cincinnati, OH.

R.L. Rolfs, P.A. Limbach (10-2006). Posttranslational and Posttranscriptional Modifications to the Large Ribosomal Subunit of Escherichia coli Grown in the Presence of Antibiotic. Oesper Symposium, University of Cincinnati, Cincinnati, OH.

M. Hossain, P.A. Limbach (10-2006). Detection of Mitochondrial Transfer RNAs Import of Saccharomyces Cerevisiae by their Signature Digestion Products. 2006 Oesper Symposium, University of Cincinnati.

A.Durairaj, P.A. Limbach (06-2006). Optimizing a Mass Spectrometry-Based Approach for the Detection of CMC-derivatized Pseudouridine in RNA. 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.

D.-M Hamburg, M.-J. Suh and P.A. Limbach (05-2006). Limited Proteolysis and Matrix-assisted Laser Desorption/Ionization Mass Spectrometry for the Interpretation of Ribosomal Subunit Topography. 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.

M. Hossain, P.A. Limbach (05-2006). Signature Digestion Product of Transfer RNA’s Advantages of Multiple Ribonucleases. 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.

S. Macha, S. Russell, L. Sallans, and P.A. Limbach (05-2006). Evaluation of In-gel Protein Digestion protocols: How Important are the Steps Involved?. 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.

S. Pourishahian, P.A. Limbach (05-2006). Characterization of Ribosomes by Chemical Cross-linking and Mass Spectrometry. 54th ASMS Conference on Mass Spectrometry and Allied Topics, Seattle, WA.

C.M Castleberry, P.A. Limbach (03-2006). Developing an LC-MS Approach for Quantifying RNAs. University of Cincinnati Graduate Poster Forum, Cincinnati, OH.

D.-M Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg (03-2006). Adventure Ribosomes Using Matrix-Assisted Laser desorption/Ionization Time-of-Flight Spectometry. University of Cincinnati Graduate Poster Forum, Cincinnati, OH.

M. Hossain P.A. Limbach (03-2006). Augmenting Transfer Ribonucleic Acids (tRNAs) Escherichia coli by using Multiple Endoribonucleases. University of Cincinnati Graduate Poster Forum, Cincinnati, OH.

D.-M Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg (03-2006). Adventures with Ribosomes Using Matrix-assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. American Chemical Society National Meeting, Atlanta, GA.

I.Nikcevic, S-H. Lee, A. Piruska, C. H. Ahn, P.A. Limbach, W. R. Heineman, C. J. Seliskar (01-2006). Characterization of Cyclic Olefin Copolymer (COC) and Poly(methylmethacrylate) (PMMA) Microchips for Capillary Electrophoresis. 10th LabAutomation 2006, Palm Springs, CA.

C. Coombs, P.A. Limbach (10-2005). Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs. Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.

A.L. Dupre, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach (10-2005). Polymer Microchips for Capillary Electrophoresis Electrospray Ionization Mass Spectrometry (CE-ESI-MS). Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.

D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (10-2005). Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Ralph and Helen Oesper Symposium, ACS Banquet and Poster Session, Cincinnati, OH.

M. Hossain, P.A. Limbach (10-2005). On the Heels of Signature Digestion Products: tRNA Treasure Hunting. Oesper Symposium, University of Cincinnati.

S. Pourshahian, M.-J. Suh, P.A. Limbach (10-2005). Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry. Oesper Symposium, University of Cincinnati.

C. M. Castleberry, P.A. Limbach (10-2005). Developing an LC-MS Approach for Quantifying RNAs. 2005 Rustbelt RNA Meeting, Deer Creek State Park & Resort, Mt. Sterling, OH.

C. M. Castleberry, P.A. Limbach (10-2005). Developing an LC-MS Approach for Quantifying RNAs. Oesper Symposium, University of Cincinnati, Cincinnati, OH.

C. Coombs, P.A. Limbach (10-2005). Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs. 2005 Rustbelt RNA Meeting, Mt. Sterling, OH.

A.Durairaj, P.A. Limbach (10-2005). Mapping Pseudouridines in RNA by MALDI Spectrometry. 2005 Rustbelt RNA Meeting, Mt. Sterling, OH.

C. Coombs, P.A. Limbach (05-2005). Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs. American Society for Mass Spectrometry, San Antonio, TX.

D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg (05-2005). Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. American Society for Mass Spectrometry, San Antonio, TX.

S. Pourshahian, M.-J. Suh, P.A. Limbach (05-2005). Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry. American Society for Mass Spectrometry, San Antonio, TX.

A.L. Dupre, W.D. Dominick, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach (03-2005). EOF Characterization of Surface-Modified PMMA Microchips for Use With Electrospray Ionization Mass Spectrometry. Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.

M. Hossain, P.A. Limbach (03-2005). A Facile Method for Detection and Quantification of Escherichia coli tRNAs by MALDI-MS. Graduate Poster Forum, University of Cincinnati, Cincinnati, OH.

A. Durairaj, K.G. Patteson, P.A. Limbach (11-2004). Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA. 2004 Rustbelt RNA Meeting, .

S. Pourshahian, M.-J. Suh, P.A. Limbach (11-2004). Investigation of the Solvent Accessible Sites of the Ribosome by Limited Proteolysis and Mass Spectrometry. 2004 Rustbelt RNA Meeting, .

P.A. Limbach, M. Hossain, Z. Meng, S. Pourshahian, M.J. Suh (06-2004). Mass Spectrometry as a Tool for Probing Ribosome Structure. Ninth Annual Meeting of the RNA Society, .

A. Dupre, W.D. Dominick, J. Mecomber, H.B. Halsall, W.R. Heineman, C.J. Seliskar, K.R. Wehmeyer, P.A. Limbach (05-2004). EOF Characterization of Surface-Modified PMMA Microchips. ALA Lab Fusion, .

C. Robins, P.A. Limbach (05-2004). Initial Ion Velocities During Charge-Transfer Ionization in MALDI-MS. 52nd ASMS Conference on Mass Spectrometry and Allied Topics, .

A. Durairaj, P.A. Limbach (05-2004). Towards Affinity Purification and Mass Spectrometric Analysis of Pseudouridines in RNA. 52nd ASMS Conference on Mass Spectrometry and Allied Topics, .

Z, Meng, M. Hossain, P.A. Limbach (05-2004). Modification Mapping, Sequencing and Quantitation of Nucleic Acids. 52nd ASMS Conference on Mass Spectrometry and Allied Topics, .

M. Tummala, L.K. Frankel, T.M. Bricker, P.A. Limbach (05-2004). Determining Protein-Protein Interactions in the Oxygen-evolving Complex (OEC) of Photosystem II by Mass Spectrometry. 52nd ASMS Conference on Mass Spectrometry and Allied Topics, .

S.B. Jayasinghe, S. Mounicou, T.D. Grant, P.A. Limbach, J.A. Caruso (05-2004). Investigation of Metalloproteins in Soy Bean Proteome by Chromatographic and Mass Spectrometric Techniques. 52nd ASMS Conference on Mass Spectrometry and Allied Topics, .

L. Jin, M. Sperling, A. Pesce, M. Wagner, P.A. Limbach (05-2004). Exploring MALDI-MS for Biomarker Screening of Colon Cancer. 52nd ASMS Conference on Mass Spectrometry and Allied Topics, .

J. Mecomber, D. Hurd, P.A. Limbach (02-2004). Rapid and Cost-Effective Prototyping of Plastic Microfabricated Devices for Mass Spectrometry. Lab Automation 2004, .

A.Durairaj, K.G. Patteson, P.A. Limbach (11-2003). Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA. 2003 Rustbelt RNA Meeting, .

M.J. Suh, S. Pourshahian, P.A. Limbach (11-2003). Developing Cross-Links and MALDI-TOFMS Approaches for Exploring Ribosomal Assembly and Interactions. 2003 Rustbelt RNA Meeting, .

P.A. Limbach, K.G. Patteson, A. Durairaj (06-2003). Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNAs. 51st ASMS Conference on Mass Spectrometry and Allied Topics, .

Z. Meng, P.A. Limbach (06-2003). Shotgun Sequencing of Modified RNAs by Nozzle-Skimmer ESI-MS. 51st ASMS Conference on Mass Spectrometry and Allied Topics, .

J.S. Mecomber, P.A. Limbach (06-2003). Rapid and Cost-effective Prototyping of Plastic Microfabricated Devices for Mass Spectrometry. 51st ASMS Conference on Mass Spectrometry and Allied Topics, .

R. Tummala, K.B. Green-Church, P.A. Limbach (05-2003). Effect of Sodium Dodecyl Sulfate on MALDI-MS Analysis of Proteolytic Digests. ”, 51st ASMS Conference on Mass Spectrometry and Allied Topics, .

M.-J. Suh, P.A. Limbach (05-2003). Developing a MALDI Mass Spectrometry Approach for Probing Ribosomal Assembly and Interactions. 51st ASMS Conference on Mass Spectrometry and Allied Topics, .

W.D. Dominick, P.A. Limbach (05-2003). Polymethylmethacrylate (PMMA) Microfabricated Devices for Mass Spectrometry. 51st ASMS Conf. on Mass Spectrometry and Allied Topics, .

W.D. Dominick, P.A. Limbach (02-2003). Surface Modification of Polymethylmethacrylate (PMMA) Microfabricated Devices for Improved Protein Characterization. Lab Automation, .

Paper Presentations

M. Hossain, P.A. Limbach. Large Scale Quantitive Analysis of Non-coding RNAs (ncRNAs) by their Signature Digestion Products using Stable Isotope Labeling and MALDI-MS. of Analytical Chemistry and Spectroscopy Societies (FACSS), Memphis, TN. 10-2007.

Kirk E. Lokits, Scott Afton, Karolin Kroening, Renee Kepler, Patrick A. Limbach and Joseph A. Caruso. ICPMS Collision Cell: Novel Applications with Xenon Gas. Federation of Analytical Chemistry and Spectroscopy Societies (FACSS), Memphis, TN. 10-2007.

C.M. Castleberry, L. Sallans, P.A. Limbach. Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY. 05-2007.

M. Hossain, P.A. Limbach. Relative Quantification of Large Ribosomal RNAs (rRNAs) by their Signature Digestion Products. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY. 05-2007.

L. Sallans, Y.B. Wetherill, J.K. Hess-Wilson, C.E.S. Comstock, S.A. Shah, C.R. Buncher, P.A. Limbach, S. Schwemberger, G.F. Babcock, and K.E. Knudsen. Bisphenol A Facilities Bypass of Androgen Ablation Therapy in Prostrate cancer: Use of Mass Spectrometry to determine Bisphenol A Levelsin the Blood Serum of Mice. Central Regional Meeting of the American Chemical Society, Northern Kentucky Convention Center, Covington, KY. 05-2007.

C.M. Castleberry, L. Sallans, P.A. Limbach. Quantitive Analysis of RNAs Using Isotope Labeling and Data-Dependent LC-MS/MS. Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH . 03-2007.

D.-M Hamburg, M.-J. Suh and P.A. Limbach. Conformational Changes and Chemical Reactivity of the Ribosome: An application of Limited Proteolysis and Matrix-assisted Laser Desorption/Ionizatization Mass Spectrometry. ”, Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH. 03-2007.

M. Hossain, P.A. Limbach. Playing SUDOKU with Nucleotides. Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH. 03-2007.

R.L. Rohlfs, P.A. Limbach. Post-translational and Post-transcriptional Modifications to the Ribosomal Subunits of Escherichia coli Grown in the presence of Antibiotic. Fourth Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH. 03-2007.

D.-M Hamburg, M.-J Suh and P.A. Limbach. Mass Spectrometry of Ribosomes for structural Elucidation. Pittcon, Chicago, IL. 02-2007.

A.Durairaj, P.A. Limbach. Optimizing a Mass Spectrometry-Based Approach for the Detection of CMC-derivatized Pseudouridine in RNA. Third Annual Mass spectrometry Symposium, Quest Conference Center, Columbus, OH. 03-2006.

C.M. Castleberry, L.Sallans, P. A. Limbach. Developing an L.C.-MS Approach for Quantifying RNAs. Third Annual Ohio Mass Spectrometry Symposium, Quest Conference Center, Columbus, OH. 03-2006.

M. Hossain, P.A. Limbach. Maximizing the Detection of transfer RNAs by Multiple Ribonucleases. Third Annual Ohio Mass Spectrometry Consortium Symposium, Quest Conference Center, Columbus, OH . 03-2006.

A.Piruska, I. Nikcevic, P. A. Limbach, W. R. Heineman, C. J. Seliskar. Optical Detection System for Multi-Lane Plastic Microchips. 10th LabAutomation 2006, Palm Springs, CA. 01-2006.

A.L. Dupre, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P. A. Limbach. Polymer Microchips for Capillary Electrophoresis Electrospray Ionization Mass Spectrometyr (CE-ESI-MS). American Institute for Chemical Engineers National Meeting, Cincinnati, OH. 11-2005.

D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg. Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. American Institute for Chemical Engineers National Meeting, Cincinnati, OH. 11-2005.

D.-M. Hamburg, P.A. Limbach, J. Carr, S. Gregory, A. Dahlberg. Analysis of Streptomycin Resistant and Dependent Thermus Thermophilus Ribosomal Protein S12 Mutants via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. 2005 Rustbelt RNA Meeting, Mt. Sterling, OH. 10-2005.

M. Hossain, P.A. Limbach. In Search of Signature Digestion Products: tRNA Treasure Hunting. 2005 Rustbelt RNA Meeting, Mt. Sterling, OH. 10-2005.

A.Piruska, I. Nikcevic, S.H. Lee, C.H. Ahn, P.A. Limbach, W.R. Heineman, C.J. Seliskar. Laser Induced Fluorescence Detection System for Multi-Lane Plastic Microchips. 230th ACS Meeting, Washington, DC. 08-2005.

C. Coombs, P.A. Limbach. Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs. Montreux LC/MS Symposium, Ithaca, NY. 07-2005.

M. Hossain, P.A. Limbach. Ribonuclease T1 Mediated Cleavage and MALDI-MS for Detection and Quantification of Escherichia coli tRNAs. American Society for Mass Spectrometry, San Antonio, TX. 05-2005.

A.L. Dupre, W.D. Dominick, J.S. Mecomber, H.B. Halsall, W.R. Heineman, K.R. Wehmeyer, P.A. Limbach. EOF Characterization of Surface-Modified PMMA Microchips for Use With Electrospray Ionization Mass Spectrometry. Ohio Mass Spectrometry Symposium, The Ohio State University, Columbus, OH . 03-2005.

D.-M. Hamburg, M.-J. Suh, P.A. Limbach, S. Gregory, A. Dahlberg. Identification of Proteins in Unsequenced Bacterial Strains Via Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry. Second Annual Mass Spectrometry Symposium, Ohio State University, Columbus, OH. 03-2005.

M. Hossain, P.A. Limbach. Detection and Quantification of Escherichia coli tRNAs by MALDI-MS. Second Annual Mass Spectrometry Symposium, Ohio State University, Columbus, OH. 03-2005.

C. Coombs, P.A. Limbach. Comparing Direct ESI-MS to LC-MS for the Analysis of Modified Nucleosides in Small RNAs. Graduate Poster Forum, University of Cincinnati, Cincinnati, OH. 03-2005.

M.J. Suh, S. Pourshahian, P.A. Limbach. Probing the Stability of Ribonucleoprotein Complexes by Mass Spectrometry. 52nd ASMS Conference on Mass Spectrometry and Allied Topics. 05-2004.

W.D. Dominick, A. Dupre, L. Jin, J. Mecomber, P.A. Limbach. Plastic Microchips for Mass Spectrometry. 52nd ASMS Conference on Mass Spectrometry and Allied Topics. 05-2004.

A. Durairaj, K.G. Patteson, P.A. Limbach. Mass Spectrometric Characterization of Pseudouridines in Ribosomal RNA. First Annual Ohio Mass Spectrometry Symposium. 03-2004.

Z. Meng, P.A. Limbach. RNase Mapping of Intact Nucleic Acids by ESI-FTICRMS and ¹⁸O Labeling. First Annual Ohio Mass Spectrometry Symposium. 03-2004.

R. Tummala, K.B. Green-Church, P.A. Limbach. Effect of Sodium Dodecyl Sulfate on MALDI-MS Analysis of Proteolytic Digests. First Annual Ohio Mass Spectrometry Symposium. 03-2004.

M.-J. Suh, P.A. Limbach. Investigation of Methods Suitable for the MALDI Mass Spectrometric Analysis of Proteins from Ribonucleoprotein Complexes. First Annual Ohio Mass Spectrometry Symposium. 03-2004.

M.J. Suh, S. Pourshahian, P.A. Limbach. Developing Cross-Linking and MALDI-TOFMS Approaches for Exploring Ribosomal Assembly and Interactions. 2003 ASMS Asilomar Conference. 10-2003.

B.T. Berhane, P.A. Limbach. Functional Microfabricated Devices for the Mass Spectral Analysis of Nucleic Acids. 51st ASMS Conference on Mass Spectrometry and Allied Topics. 06-2003.

S.N. Mounicou, J. Meija, P.A. Limbach, J.A. Caruso. The Synergy of Tandem Atomic and Molecular Mass Spectrometry Approaches for Characterizing Metalloproteins. 51st ASMS Conference on Mass Spectrometry and Allied Topics. 06-2003.

C. Robins, P.A. Limbach. Adding Selectivity to MALDI-MS for Nonpolar Analytes. The 30th Annual Conference of NOBCChE. 03-2003.

Honors & Awards

Fellow of the Graduate School, University of Cincinnati.

Ohio Eminent Scholar in Mass Spectrometry.

Sigma Xi Research Recognition Award.